Role of Acinetobacter baumannii in decolorization of reactive blue 224 dye and functional analysis of azoreductase gene
Bioremediation is a sustainable and worthy approach for remediating textile industrial wastewater, which poses severe extortions to human and environmental health due to its toxic, mutagenic, and carcinogenic nature. The present study employed Acinetobacter baumannii 1005 isolated from industrial wa...
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Elsevier B.V.
2025
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| author | Ullah F. Mustafa G. Tariq Zahid M. Jamil I. Zaghum Abbas S. Jeon B.-H. Alessa A.H. Rafatullah M. |
| author2 | 58400094300 |
| author_facet | 58400094300 Ullah F. Mustafa G. Tariq Zahid M. Jamil I. Zaghum Abbas S. Jeon B.-H. Alessa A.H. Rafatullah M. |
| author_sort | Ullah F. |
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| content_provider | Universiti Tenaga Nasional |
| content_source | UNITEN Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Bioremediation is a sustainable and worthy approach for remediating textile industrial wastewater, which poses severe extortions to human and environmental health due to its toxic, mutagenic, and carcinogenic nature. The present study employed Acinetobacter baumannii 1005 isolated from industrial wastewater to emphasize its degradation potential and to characterize the azoreductase (AZA) gene to assess and optimize the biodegradation of reactive blue 224 (RB-224) textile dye. A. baumannii showed significant decolorization against RB-224, resulting in a change in color. Under optimized conditions (pH 6, 37 �C, and 100 mg/L), A. baumannii exhibited 91 % decolorization of RB-224 being analyzed by UV?vis after 24 h of incubation. UV?vis spectroscopy and FTIR analysis have shown the efficiency of A. baumannii for degrading RB-224. In the UV spectrum, the shifting of the peak from560 nm to 400 nm confirmed the decolorization. In the FTIR spectrum, the shift of several significant peaks related to the functional groups S = O C-N, C?H, C = C, and N?H, corresponding to the structure of RB-224 dye along with the emergence of new peaks indicating the formation of metabolites after degradation. The Azoreductase (AZA) gene of ?600 bp was amplified and cloned in a pTz57R/T cloning vector. Subsequently, the AZA gene was sequenced and submitted to NCBI for accession number. This study explored the potential of A. baumannii isolate suitable for the bio-remediation of textile dyes. ? 2024 The Author(s) |
| format | Article |
| id | my.uniten.dspace-36449 |
| institution | Universiti Tenaga Nasional |
| publishDate | 2025 |
| publisher | Elsevier B.V. |
| record_format | dspace |
| spelling | my.uniten.dspace-364492025-03-03T15:42:29Z Role of Acinetobacter baumannii in decolorization of reactive blue 224 dye and functional analysis of azoreductase gene Ullah F. Mustafa G. Tariq Zahid M. Jamil I. Zaghum Abbas S. Jeon B.-H. Alessa A.H. Rafatullah M. 58400094300 59423870200 57215589901 59153060700 59152489700 57653536800 57981906100 15050908200 Bioremediation is a sustainable and worthy approach for remediating textile industrial wastewater, which poses severe extortions to human and environmental health due to its toxic, mutagenic, and carcinogenic nature. The present study employed Acinetobacter baumannii 1005 isolated from industrial wastewater to emphasize its degradation potential and to characterize the azoreductase (AZA) gene to assess and optimize the biodegradation of reactive blue 224 (RB-224) textile dye. A. baumannii showed significant decolorization against RB-224, resulting in a change in color. Under optimized conditions (pH 6, 37 �C, and 100 mg/L), A. baumannii exhibited 91 % decolorization of RB-224 being analyzed by UV?vis after 24 h of incubation. UV?vis spectroscopy and FTIR analysis have shown the efficiency of A. baumannii for degrading RB-224. In the UV spectrum, the shifting of the peak from560 nm to 400 nm confirmed the decolorization. In the FTIR spectrum, the shift of several significant peaks related to the functional groups S = O C-N, C?H, C = C, and N?H, corresponding to the structure of RB-224 dye along with the emergence of new peaks indicating the formation of metabolites after degradation. The Azoreductase (AZA) gene of ?600 bp was amplified and cloned in a pTz57R/T cloning vector. Subsequently, the AZA gene was sequenced and submitted to NCBI for accession number. This study explored the potential of A. baumannii isolate suitable for the bio-remediation of textile dyes. ? 2024 The Author(s) Final 2025-03-03T07:42:29Z 2025-03-03T07:42:29Z 2024 Article 10.1016/j.jksus.2024.103279 2-s2.0-85194905858 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85194905858&doi=10.1016%2fj.jksus.2024.103279&partnerID=40&md5=74d20bb6988ad81cb89496d93e203675 https://irepository.uniten.edu.my/handle/123456789/36449 36 7 103279 All Open Access; Gold Open Access Elsevier B.V. Scopus |
| spellingShingle | Ullah F. Mustafa G. Tariq Zahid M. Jamil I. Zaghum Abbas S. Jeon B.-H. Alessa A.H. Rafatullah M. Role of Acinetobacter baumannii in decolorization of reactive blue 224 dye and functional analysis of azoreductase gene |
| title | Role of Acinetobacter baumannii in decolorization of reactive blue 224 dye and functional analysis of azoreductase gene |
| title_full | Role of Acinetobacter baumannii in decolorization of reactive blue 224 dye and functional analysis of azoreductase gene |
| title_fullStr | Role of Acinetobacter baumannii in decolorization of reactive blue 224 dye and functional analysis of azoreductase gene |
| title_full_unstemmed | Role of Acinetobacter baumannii in decolorization of reactive blue 224 dye and functional analysis of azoreductase gene |
| title_short | Role of Acinetobacter baumannii in decolorization of reactive blue 224 dye and functional analysis of azoreductase gene |
| title_sort | role of acinetobacter baumannii in decolorization of reactive blue 224 dye and functional analysis of azoreductase gene |
| url_provider | http://dspace.uniten.edu.my/ |