Selection of acid salts: A critical step in creating an acidic condition for plasma iron release and measurement
Introduction: Iron deficiency anaemia (IDA) is the most common cause of anaemia worldwide. Determination of body iron status is necessary to diagnose IDA. This can be measured using a biochemistry assessment of the serum/ plasma. Plasma/serum iron quantitation is also important in diagnosing iron ov...
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Universiti Putra Malaysia Press
2023
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| author | Lim W.F. Abdullah M. Ho K.L. Yap B.K. Lai M.I. |
| author2 | 50061393300 |
| author_facet | 50061393300 Lim W.F. Abdullah M. Ho K.L. Yap B.K. Lai M.I. |
| author_sort | Lim W.F. |
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| content_provider | Universiti Tenaga Nasional |
| content_source | UNITEN Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Introduction: Iron deficiency anaemia (IDA) is the most common cause of anaemia worldwide. Determination of body iron status is necessary to diagnose IDA. This can be measured using a biochemistry assessment of the serum/ plasma. Plasma/serum iron quantitation is also important in diagnosing iron overload disorders. However, iron studies are limited due to high cost and lack of access to biochemical analysers. Therefore, a cost- and technical-effective method is needed to measure human plasma iron concentration. Plasma iron is mainly transferrin-bound and an acidic plasmic condition is necessary to release the iron. This study investigated various candidate acid salts to achieve the acidic condition needed for plasma iron release. Method: Ten powdered or crystallised acid salts were studied for their water solubility as well as their pH reduction capability in revised simulated body fluid (r-SBF) and commercially available human plasma without any change in colour or form. Results: Six acid salts studied were discontinued from further investigation because they were insoluble in water. Another two candidates were unsuitable as they precipitated in r-SBF and human plasma. Maleic acid formed a jelly-like texture after a certain amount of time in human plasma. Only citric acid met all the criteria of a suitable acid salt to be investigated further as part of the reagent for a spontaneous plasma iron measurement. Conclusion: Citric acid, which is a colourless and odourless acid salt, was selected to lower the human plasma pH to an acidic condition for transferrin-bound iron release. � 2020 UPM Press. All rights reserved. |
| format | Article |
| id | my.uniten.dspace-25170 |
| institution | Universiti Tenaga Nasional |
| publishDate | 2023 |
| publisher | Universiti Putra Malaysia Press |
| record_format | dspace |
| spelling | my.uniten.dspace-251702023-05-29T16:07:06Z Selection of acid salts: A critical step in creating an acidic condition for plasma iron release and measurement Lim W.F. Abdullah M. Ho K.L. Yap B.K. Lai M.I. 50061393300 55666914500 18233493700 26649255900 13605357700 Introduction: Iron deficiency anaemia (IDA) is the most common cause of anaemia worldwide. Determination of body iron status is necessary to diagnose IDA. This can be measured using a biochemistry assessment of the serum/ plasma. Plasma/serum iron quantitation is also important in diagnosing iron overload disorders. However, iron studies are limited due to high cost and lack of access to biochemical analysers. Therefore, a cost- and technical-effective method is needed to measure human plasma iron concentration. Plasma iron is mainly transferrin-bound and an acidic plasmic condition is necessary to release the iron. This study investigated various candidate acid salts to achieve the acidic condition needed for plasma iron release. Method: Ten powdered or crystallised acid salts were studied for their water solubility as well as their pH reduction capability in revised simulated body fluid (r-SBF) and commercially available human plasma without any change in colour or form. Results: Six acid salts studied were discontinued from further investigation because they were insoluble in water. Another two candidates were unsuitable as they precipitated in r-SBF and human plasma. Maleic acid formed a jelly-like texture after a certain amount of time in human plasma. Only citric acid met all the criteria of a suitable acid salt to be investigated further as part of the reagent for a spontaneous plasma iron measurement. Conclusion: Citric acid, which is a colourless and odourless acid salt, was selected to lower the human plasma pH to an acidic condition for transferrin-bound iron release. � 2020 UPM Press. All rights reserved. Final 2023-05-29T08:07:06Z 2023-05-29T08:07:06Z 2020 Article 2-s2.0-85099388878 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85099388878&partnerID=40&md5=9aa54e9b0978a7ee3f13ff4f996c9c79 https://irepository.uniten.edu.my/handle/123456789/25170 16 52 57 Universiti Putra Malaysia Press Scopus |
| spellingShingle | Lim W.F. Abdullah M. Ho K.L. Yap B.K. Lai M.I. Selection of acid salts: A critical step in creating an acidic condition for plasma iron release and measurement |
| title | Selection of acid salts: A critical step in creating an acidic condition for plasma iron release and measurement |
| title_full | Selection of acid salts: A critical step in creating an acidic condition for plasma iron release and measurement |
| title_fullStr | Selection of acid salts: A critical step in creating an acidic condition for plasma iron release and measurement |
| title_full_unstemmed | Selection of acid salts: A critical step in creating an acidic condition for plasma iron release and measurement |
| title_short | Selection of acid salts: A critical step in creating an acidic condition for plasma iron release and measurement |
| title_sort | selection of acid salts: a critical step in creating an acidic condition for plasma iron release and measurement |
| url_provider | http://dspace.uniten.edu.my/ |