The population of airborne microorganisms and the presence of Staphylococcus aureus in cattle farm at Ladang Pasir Akar
Microorganisms found in bio aerosols from animals’ confinement buildings is not only foster the risk of spreading diseases among livestock itself, but also pose health hazard among farm workers and nearby residents. The objectives of this study are to observe the population of microorganisms and i...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | en en |
| Published: |
Penerbit Universiti Sultan Zainal Abidin
2020
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| Subjects: | |
| Online Access: | http://eprints.unisza.edu.my/7270/1/FH02-FBIM-20-43238.pdf http://eprints.unisza.edu.my/7270/2/FH02-FBIM-21-51315.pdf http://eprints.unisza.edu.my/7270/ |
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| Summary: | Microorganisms found in bio aerosols from animals’ confinement buildings is not only foster the risk of
spreading diseases among livestock itself, but also pose health hazard among farm workers and nearby residents.
The objectives of this study are to observe the population of microorganisms and identify the present of
Staphylococcus aureus (S. aureus) inside the cattle farm. DRF e-MAS which was owned prepared by Arduino kit had
been used to observe the microorganisms’ population.The air from surrounding that contained microorganisms
being aspirated into the plate chamber of the DRF e-MAS. The nutrient agar that being placed inside the plate
chamber will be incubated along with aspirated microorganisms and the result of microorganisms’ population
will be observed. S. aureus is one of the possible bacteria that may be found among the population of the
microorganisms in cattle farm. DRF e-MAS along with Baird Parker Agar being used to identify the present of
S. aureus. Baird Parker Agar is used to enhance the growth of S. aureus while eliminating the others. After being
incubated, a single colony of S. aureus that grew on the Baird Parker Agar had been picked and regrow on
another nutrient agar. A single colony that formed on that nutrient agar will be picked and then undergo
Coagulase test and IMViC test. Coagulase test involved the citrated plasma and the present of bubbles show
positive result. On the other hand, IMViC test divided into four sub-test which are Indole test, Methyl red test,
Voges-Proskauer test and Citrate test. These tests are important in order to confirm the present of S. aureus in
this farm. According to the test, there was S. aureus found among the microorganisms’ population inside this
barn. |
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