In Vitro Micropropagation of Aquarium Plants Pearl Grass Hemianthus micranthemoides (Nuttall) and Micro Sword Grass Lilaeopsis brasiliensis (Glaziou) Affolter (Apiaceae)
Hemianthus micranthemoides and Lileaopsis brasiliensis are nowadays sold in the form of tissue culture production by aquaria and ornamental trade in Malaysia. However, there is no to limited report about in vitro micropropagation of both aquarium plants. In this study, an experimental procedure fo...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | en |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://eprints.unisza.edu.my/6573/1/FH02-FBIM-19-33507.pdf http://eprints.unisza.edu.my/6573/ |
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| Summary: | Hemianthus micranthemoides and Lileaopsis brasiliensis are nowadays sold in the form of tissue culture production by
aquaria and ornamental trade in Malaysia. However, there is no to limited report about in vitro micropropagation
of both aquarium plants. In this study, an experimental procedure for micropropagation of both plants was
documented. The propagation were done in Murashige and Skoog (MS) medium with eight combination of
different concentration of 6-Benzylaminopurine with Naphteneacetic acid (BAP-NAA) and 1-phenyl-3-1,2,3-
thiadizol-5-yl urea with Naphteneacetic acid (TDZ-NAA) following the rooting technique for 42 days. All
treatments for 1 L MS medium were regulated to pH 5.7 to 5.8 and added with sucrose (30 g/L) and phytagel (2.5
g/L). All combination treatments had induced rooting. There was a significance different in the shoot regeneration
of H. micranthemoides in all treatment (95% confidence level, F = 13.907, n = 240, p = 0.000), on the contrary there
was no different were observed in shoot regeneration of L. brasiliensis treatment. Overall, all treatment of TDZNAA were significantly higher in the shoot regeneration than those in BAP-NAA treatment. Particularly the T7
(TDZ-NAA, 0.075: 0.1 mg/L) given highest result in diameter (1.626±0.213 cm) and area (1.724±0.308 cm2) of
shoot clumps in H. micranthemoides, and formation of shoots (51.5±4.95), nodes (20.0±2.83) and roots (45.5±13.4)
in L. brasiliensis. Hardening of in vitro cultured plantlets was done in aquatic laboratory. All of the plants survived
and transferred into aquarium. This study has provided beneficial information in further micropropagation
protocol for the mass production of both aquarium plants. |
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