Construction of single-chain variable fragment antibodies against MCF-7 breast cancer cells
A phage display library of single chain variable fragment (scFv) against MCF-7 breast cancer cells was constructed from C3A8 hybridoma cells . RNA from the C3A8 was isolated, cDNA was constructed, and variable heavy and light immunoglobulin chain gene region were amplified using PCR. The variable...
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| Main Authors: | , |
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| Format: | Article |
| Language: | en en |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://eprints.unisza.edu.my/2663/1/FH02-FBIM-19-25212.pdf http://eprints.unisza.edu.my/2663/2/FH02-FBIM-19-25348.pdf http://eprints.unisza.edu.my/2663/ |
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