Construction of single-chain variable fragment antibodies against MCF-7 breast cancer cells

A phage display library of single chain variable fragment (scFv) against MCF-7 breast cancer cells was constructed from C3A8 hybridoma cells . RNA from the C3A8 was isolated, cDNA was constructed, and variable heavy and light immunoglobulin chain gene region were amplified using PCR. The variable...

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Bibliographic Details
Main Authors: Abd Manaf, Ali, Noor Asidah, Mohamed
Format: Article
Language:en
en
Published: 2013
Subjects:
Online Access:http://eprints.unisza.edu.my/2663/1/FH02-FBIM-19-25212.pdf
http://eprints.unisza.edu.my/2663/2/FH02-FBIM-19-25348.pdf
http://eprints.unisza.edu.my/2663/
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