Effect of Glycosaminoglycans Extract From Sea Cucumber Stichopus vastus on Inflammatory Mediators and Tissue Healing on Rodents With Full Thickness Burn Injury
This study was design to investigate the effect of total sulfated GAG from integument body wall of sea cucumber Stichopus vastus on inflammatory mediators and tissue healing on rodents with full thickness burn injury. Twelve male Sprague-dawley rats weighting 350-400 grams were distributed random...
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| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | en |
| Published: |
2017
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| Subjects: | |
| Online Access: | http://eprints.unisza.edu.my/1449/1/FH03-FP-17-10517.pdf http://eprints.unisza.edu.my/1449/ |
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| Summary: | This study was design to investigate the effect of total sulfated GAG from
integument body wall of sea cucumber Stichopus vastus on inflammatory mediators and
tissue healing on rodents with full thickness burn injury. Twelve male Sprague-dawley rats
weighting 350-400 grams were distributed randomly into two groups. Six rats in group A
received 5 ml/kg/day intraperitoneal total sulfated GAG from S.vastus for 5 days, while
control group received phosphate buffered saline (PBS). Level of IL-10 and IL-12 were
measured at day 1, 6 and 12. The level of IL-10 revealed significantly (p<0.05) higher and
the level of IL-12 was reduced significantly (p<0.05) in sulfated GAG treated group. Data
suggested that sulfated GAG from S. vastus integument body wall increase antiinflammatory cytokine and reduce pro-inflammatory cytokine. Microscopic evaluation
showed reduced neutrophils infiltration with enhanced proliferation of fibroblasts and
angiogenesis (p<0.05) in the sulfated GAG treated group. Sulfated GAG from S. vastus
integument body wall has impact on the outcome of wound healing process by increasing
the level of anti-inflammatory cytokine, IL-10 and reduced level of pro-inflammatory
cytokine, IL-12 and positive effect on fibroblast proliferation and angiogenesis process. |
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