Lignin biodegradation and ligninolytic enzyme studies during biopulping of Acacia Mangium wood chips by tropical white rot fungi
Abstract White rot fungi are good lignin degraders and have the potential to be used in industry. In the present work, Phellinus sp., Daedalea sp., Trametes versicolor and Pycnoporus coccineus were selected due to their relatively high ligninolytic enzyme activity, and grown on Acacia mangium w...
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| Format: | Article |
| Language: | en |
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Springer Link
2011
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| Online Access: | http://ir.unimas.my/id/eprint/9607/7/Lignin.pdf http://ir.unimas.my/id/eprint/9607/ http://link.springer.com/article/10.1007%2Fs11274-010-0598-x |
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| _version_ | 1831806079187550208 |
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| author | C.Y, Liew A., Husaini H., Hussain S., Muid K.C., Liew H.A., Roslan |
| author_facet | C.Y, Liew A., Husaini H., Hussain S., Muid K.C., Liew H.A., Roslan |
| author_sort | C.Y, Liew |
| building | Centre for Academic Information Services (CAIS) |
| collection | Institutional Repository |
| content_provider | Universiti Malaysia Sarawak |
| content_source | UNIMAS Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Abstract White rot fungi are good lignin degraders and
have the potential to be used in industry. In the present
work, Phellinus sp., Daedalea sp., Trametes versicolor and
Pycnoporus coccineus were selected due to their relatively
high ligninolytic enzyme activity, and grown on Acacia
mangium wood chips under solid state fermentation.
Results obtained showed that manganese peroxidase produced
is far more compared to lignin peroxidase, suggesting
that MnP might be the predominating enzymes
causing lignin degradation in Acacia mangium wood chips.
Cellulase enzyme assays showed that no significant cellulase
activity was detected in the enzyme preparation of
T. versicolor and Phellinus sp. This low cellulolytic
activity further suggests that these two white rot strains are
of more interest in lignin degradation. The results on lignin
losses showed 20–30% of lignin breakdown at 60 days of
biodegradation. The highest lignin loss was found in Acacia
mangium biotreated with T. versicolor after 60 days and recorded 26.9%, corresponding to the percentage of
their wood weight loss recorded followed by P. coccineus.
In general, lignin degradation was only significant from
20 days onwards. The overall percentage of lignin weight
loss was within the range of 1.02–26.90% over the biodegradation
periods. Microscopic observations conducted
using scanning electron microscope showed that T. versicolor,
P. coccineus, Daedalea sp. and Phellinus sp. had
caused lignin degradation in Acacia mangium wood chips. |
| format | Article |
| id | my.unimas.ir-9607 |
| institution | Universiti Malaysia Sarawak |
| language | en |
| publishDate | 2011 |
| publisher | Springer Link |
| record_format | eprints |
| spelling | my.unimas.ir-96072022-08-10T07:41:29Z http://ir.unimas.my/id/eprint/9607/ Lignin biodegradation and ligninolytic enzyme studies during biopulping of Acacia Mangium wood chips by tropical white rot fungi C.Y, Liew A., Husaini H., Hussain S., Muid K.C., Liew H.A., Roslan SD Forestry Abstract White rot fungi are good lignin degraders and have the potential to be used in industry. In the present work, Phellinus sp., Daedalea sp., Trametes versicolor and Pycnoporus coccineus were selected due to their relatively high ligninolytic enzyme activity, and grown on Acacia mangium wood chips under solid state fermentation. Results obtained showed that manganese peroxidase produced is far more compared to lignin peroxidase, suggesting that MnP might be the predominating enzymes causing lignin degradation in Acacia mangium wood chips. Cellulase enzyme assays showed that no significant cellulase activity was detected in the enzyme preparation of T. versicolor and Phellinus sp. This low cellulolytic activity further suggests that these two white rot strains are of more interest in lignin degradation. The results on lignin losses showed 20–30% of lignin breakdown at 60 days of biodegradation. The highest lignin loss was found in Acacia mangium biotreated with T. versicolor after 60 days and recorded 26.9%, corresponding to the percentage of their wood weight loss recorded followed by P. coccineus. In general, lignin degradation was only significant from 20 days onwards. The overall percentage of lignin weight loss was within the range of 1.02–26.90% over the biodegradation periods. Microscopic observations conducted using scanning electron microscope showed that T. versicolor, P. coccineus, Daedalea sp. and Phellinus sp. had caused lignin degradation in Acacia mangium wood chips. Springer Link 2011 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/9607/7/Lignin.pdf C.Y, Liew and A., Husaini and H., Hussain and S., Muid and K.C., Liew and H.A., Roslan (2011) Lignin biodegradation and ligninolytic enzyme studies during biopulping of Acacia Mangium wood chips by tropical white rot fungi. World Journal of Microbiology and Biotechnology, 27 (6). pp. 1457-1468. http://link.springer.com/article/10.1007%2Fs11274-010-0598-x DOI 10.1007/s11274-010-0598-x |
| spellingShingle | SD Forestry C.Y, Liew A., Husaini H., Hussain S., Muid K.C., Liew H.A., Roslan Lignin biodegradation and ligninolytic enzyme studies during biopulping of Acacia Mangium wood chips by tropical white rot fungi |
| title | Lignin biodegradation and ligninolytic enzyme studies
during biopulping of Acacia Mangium wood chips by tropical white rot fungi |
| title_full | Lignin biodegradation and ligninolytic enzyme studies
during biopulping of Acacia Mangium wood chips by tropical white rot fungi |
| title_fullStr | Lignin biodegradation and ligninolytic enzyme studies
during biopulping of Acacia Mangium wood chips by tropical white rot fungi |
| title_full_unstemmed | Lignin biodegradation and ligninolytic enzyme studies
during biopulping of Acacia Mangium wood chips by tropical white rot fungi |
| title_short | Lignin biodegradation and ligninolytic enzyme studies
during biopulping of Acacia Mangium wood chips by tropical white rot fungi |
| title_sort | lignin biodegradation and ligninolytic enzyme studies
during biopulping of acacia mangium wood chips by tropical white rot fungi |
| topic | SD Forestry |
| url | http://ir.unimas.my/id/eprint/9607/7/Lignin.pdf http://ir.unimas.my/id/eprint/9607/ http://link.springer.com/article/10.1007%2Fs11274-010-0598-x |
| url_provider | http://ir.unimas.my/ |