The antibiotic resistance profile of Enterobacteriaceae isolated from salt licks within Baleh and Ulu Kapit regions in Sarawak
Aims: This study primarily reports the isolation and identification of antibiotic-resistant Enterobacteriaceae from salt licks in the interior parts of Baleh in Kapit District, Sarawak, Malaysia. Methodology and results: Thirty Enterobacteriaceae isolates were identified by 16S rRNA sequencing. The...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | en |
| Published: |
Malaysian Society for Microbiology
2025
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/49158/1/EV%20Formatted-%20MJM2-23-0517-Final.pdf http://ir.unimas.my/id/eprint/49158/ https://mjm.usm.my/uploads/issues/2086/EV%20Formatted-%20MJM2-23-0517-Colour%20Ready.pdf http://dx.doi.org/10.21161/mjm.230517 |
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| Summary: | Aims: This study primarily reports the isolation and identification of antibiotic-resistant Enterobacteriaceae from salt licks in the interior parts of Baleh in Kapit District, Sarawak, Malaysia.
Methodology and results: Thirty Enterobacteriaceae isolates were identified by 16S rRNA sequencing. The 16S rRNA
sequence based identification of isolates revealed the presence of Escherichia coli (48%, n=16), Enterobacter soli (9%,n=3), Citrobacter amalonaticus (9%, n=3), E. lignolyticus (6%, n=2), E. albertii (6%, n=2) and the remaining each with a percentage of 3% (n=1) were Enterobacter sp., Kluyvera ascorbata, C. freundii and Citrobacter sp., respectively. Based on the antimicrobial analysis, all isolates (100%, n=30) were sensitive to four of the antibiotics tested, namely ceftazidime, gentamicin, ofloxacin and tetracycline. Higher susceptibility was also observed against trimethoprim (86.7%, n=26) and nalidixic acid (63.3%, n=19). In contrast, the isolates showed low resistant activities towards kanamycin (40%, n=12) and trimethoprim (3.3%, n= 1). Additionally, the isolates were observed to show intermediate against kanamycin
(60%, n=18), nalidixic acid (36.7%, n=11) and trimethoprim (10%, n=3). MAR index of all the isolates (100%) was below
0.2, indicating that these isolates originate low risk of antibiotic contamination sources.
Conclusion, significance and impact of study: Despite the low level of antibiotic-resistant of the Enterobacteriaceae
from the salt licks, there is still a possibility that the transmission of the antibiotic-resistant genes among bacterial communities in the salt lick environment. The findings of this study provide valuable insight into the conservation effort of salt lick resources. |
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