Feasibility of Sago bioethanol liquid waste as a feedstock for laccase production in recombinant Pichia pastoris
The use of waste stream generated upon the production of bioethanol from sago fibre has not been a major focus in the literature thus far. This study explores the feasibility of utilising the liquid waste generated following the production of bioethanol from sago fibre as a feedstock for the pr...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | en |
| Published: |
World Research Associations
2021
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/47823/1/Feasibility%20of%20Sago.pdf http://ir.unimas.my/id/eprint/47823/ https://www.researchgate.net/publication/353066745_Feasibility_of_Sago_bioethanol_liquid_waste_as_a_feedstock_for_laccase_production_in_recombinant_Pichia_pastoris |
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| Summary: | The use of waste stream generated upon the production
of bioethanol from sago fibre has not been a major
focus in the literature thus far. This study explores the
feasibility of utilising the liquid waste generated
following the production of bioethanol from sago fibre
as a feedstock for the production of recombinant
laccase in Pichia pastoris GS115. Characterisation of
the sago bioethanol liquid waste (SBLW) indicated that
glycerol was the main constituent along with glucose
and lactic acid. Our results showed that P. pastoris
GS115 growth was generally feasible when SBLW was
used as a feedstock irrespective of its concentration.
The expression of laccase reached the highest in
fermentations that employed 40% (v/v) SBLW, whilst
higher concentrations of SBLW resulted in the
reduction of the laccase activity. The highest laccase
activity achieved using 40% (v/v) SBLW represented
73% of that obtained using the standard synthetic
medium. Further enhancement of maximum biomass
concentration by 1.2-fold and laccase titre by 1.5-fold
was achieved when the 40% (v/v) SBLW was
supplemented with 1.0% (w/v) yeast extract. This work
gives useful insights into the promising applications of
SBLW as an inexpensive and sustainable feedstock for
the production of industrial biocatalysts. |
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