Genetics and Morphological Variations within Genus Callosciurus from Borneo
Callosciurus, which is a genus of squirrels that fall under the Family Sciuridae, is an example of Southeast Asia's mammalian group with great species diversity. However, precise genetic study on the Sciuridae is finite, apart from a few recent studies with limited sample site from Borneo. Th...
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| Format: | Final Year Project Report / IMRAD |
| Language: | en |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2022
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/39701/2/M%20Hanif%20Fikri.pdf http://ir.unimas.my/id/eprint/39701/ |
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| Summary: | Callosciurus, which is a genus of squirrels that fall under the Family Sciuridae, is an example of Southeast Asia's
mammalian group with great species diversity. However, precise genetic study on the Sciuridae is finite, apart
from a few recent studies with limited sample site from Borneo. The main purpose of this study is to analyze the
genetics and morphological variation within genus Callosciurus from Borneo. In this study, secondary data of
Callosciurus from the museum of Faculty of Resource Science and Technology UNIMAS were being used. A
total of 27 sequences were utilized to build a phylogenetic tree using neighbor-joining, maximum parsimony, and
maximum likelihood methods. Sequence from GenBank were also used in this study to get the DNA sequence
information of Callosciurus that are not available at the museum. Cytochrome c oxidase I (COI) were used as
genetic marker with VF1 forward and VR1 reverse as primer. For morphological analysis, Principal Component
Analysis (PCA) was conducted by using PAST software, using 24 individuals of Callosciurus with five
morphometric characteristics based on external morphology. Results from this study declared that with high
bootstrap value from phylogenetic analysis, the connection of the genus Callosciurus showed the existence of
genetic divergence within this genus, and the genetic study result indicated that one sample from the museum had
been misidentified as Callosciurus notatus. From the morphological analysis, the findings revealed that their
morphometrics differ, and the species may be recognized by comparing tail to ventral length and head to body
length. This proved that there is significant difference in genetic and morphological variation within the genus
Callosciurus from Borneo. |
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