Rapid isolation and detection of Escherichia coli O157:H7 by use of rainbow agar O157 and PCR assay
This study has evaluated the use of a commercially available Rainbow agar O157 and polymerase chain reaction (PCR) assays for the detection of Shiga-like toxin producing Escherichia coli and to serotype E. coli O157:H7 from raw meat. The Rainbow agar O157 was found to be selective and sensitive for...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | en |
| Published: |
The Southeast Asian Journal of Tropical Medicine and Public Health SEAMEO
2000
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/39253/3/RAPID%20ISOLATION%20-%20Copy.pdf http://ir.unimas.my/id/eprint/39253/ https://pubmed.ncbi.nlm.nih.gov/11023069/ |
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| Summary: | This study has evaluated the use of a commercially available Rainbow agar O157 and polymerase chain reaction (PCR) assays for the detection of Shiga-like toxin producing Escherichia coli and to serotype E. coli O157:H7 from raw meat. The Rainbow agar O157 was found to be selective and sensitive for the screening of the E. coli O157 from artificially and naturally contaminated meat samples. Shiga-like toxin producing E. coli were identified with two primer pairs that amplified fragments of the SLT-I (384 bp) and SLT-II (584 bp). E. coli O157:H7 was serotyped with a primer pair specified for the H7 flagellar gene, which amplify specific DNA fragments (625 bp) from all E. coli O157:H7 strains. The use of Rainbow agar O157 described allows for the presumptive isolation of E. coli O157 in 24 hours. Identification and confirmation of the presumptive isolates as E. coli O157:H7 by PCR assays require additional 6-8 hours. The above-mentioned screening and identification procedures should prove to be a very useful method since it allows for the specific detection of E. coli O157:H7. |
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