Tissue Culture Studies on Fortunella polyandra ‘Nagami’ and ‘Meiwa’
Studies of Fortunella polyandra ‘Nagami’ and ‘Meiwa’ were carried out to observe their responses in tissue culture systems. Explant sources investigated included roots, stems, leaves and cotyledons which were cultured on Murashige and Skoog (MS) medium supplemented with different combinations and...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | en |
| Published: |
ISHS
2013
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/36209/1/polyandra1.pdf http://ir.unimas.my/id/eprint/36209/ https://www.actahort.org/books/975/975_38.htm |
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| Summary: | Studies of Fortunella polyandra ‘Nagami’ and ‘Meiwa’ were carried out to
observe their responses in tissue culture systems. Explant sources investigated
included roots, stems, leaves and cotyledons which were cultured on Murashige and
Skoog (MS) medium supplemented with different combinations and concentrations of
various hormones. The hormones used were naphthalene acetic acid (NAA) and
benzylaminopurine (BAP) at pH 5.8 with a temperature of 23-26°C and a photoperiod
of 16 hours light and 8 hours dark. The pH of the media was also altered to include
4.8, 5.8, 6.8 and 7.8. The various explants were subjected to light and dark treatments
in order to study the morphogenesis of this species. From the results, it was observed
that stem and leaf explants were more responsive than other explants. The best media
for regeneration and callus formation was MS supplemented with 0.5 mg/L NAA and
0.5 mg/L BAP at pH 5.8 for ‘Nagami’ and pH 7.8 for ‘Meiwa’. Regeneration was
achieved via direct organogenesis and also via callus formation. The highest
percentage of shoot formation (40%) was obtained from stem explants (‘Meiwa’)
cultured on MS supplemented with 0.5 mg/L NAA and 0.5 mg/L BAP at pH 7.8. |
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