In vitro regeneration and multiplication of borneo's endemic orchid phalaenopsis
Orchids are the second largest families of flowering plant after sunflowers families. Phalaenopsis and Vanda are the most popular orchids as potted plant and cut flowers across the world. Both of these species are endemic to Borneo and have been listed as endangered species. In vitro propagation...
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| Format: | Final Year Project Report / IMRAD |
| Language: | en |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2015
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/34112/2/In%20vitro%20regeneration%20and%20multiplication%20of%20borneo%27s%20endemic%20orchid%20phalaenopsis%28fulltext%29.pdf http://ir.unimas.my/id/eprint/34112/ |
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| Summary: | Orchids are the second largest families of flowering plant after sunflowers families.
Phalaenopsis and Vanda are the most popular orchids as potted plant and cut flowers across
the world. Both of these species are endemic to Borneo and have been listed as endangered
species. In vitro propagation is the technique used in this study. The objective of this study
was to determine the effects of plant growth regulators and organic additives on
multiplication of Phalaenopsis and Vanda orchids. The protocorms of Vanda dearei was
cultured on½ MS media with the supplement of plant growth regulators, NAA and BAP and
organic compound, coconut water, potato homogenate and banana homogenate at different
concentrations. Between NAA and BAP, 0.5 mg/L of NAA has shown the most effective
proliferation ofprotocorm with an average of 11.72. For organic additives, the most effective
proliferation of protocorm was 15% of coconut water with an average of 8.32 number of
new protocorms formed. For Phalaenopsis bellina, explants were culture on NAA, BAP and
TDZ at different concentration, only 1.0 mg/L of TDZ has shown spherical like body formed
after 12 weeks of culture. |
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