Isolation and Characterization of Antibiotic Resistant Bacteria from Swiftlet Feces in Swiftlet Farm Houses in Sarawak, Malaysia
There is a growing concern on the occurrence of antimicrobial resistance. Development of multiple antibiotic resistant bacteria has overtaken new drug development and threatened the patients with untreatable infections. This study was conducted to isolate and characterize the antibiotic resistant...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | en |
| Published: |
Indonesian Society for Microbiology (Secretariat PERMI)
2013
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/31025/1/Leong.pdf http://ir.unimas.my/id/eprint/31025/ https://jurnal.permi.or.id/index.php/mionline/article/view/231 |
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| Summary: | There is a growing concern on the occurrence of antimicrobial resistance. Development of multiple
antibiotic resistant bacteria has overtaken new drug development and threatened the patients with untreatable
infections. This study was conducted to isolate and characterize the antibiotic resistant bacteria from swiftlet farm houses located in various places including Kota Samarahan, Semarang, Saratok, Betong, Sarikei, Sibu,
Sepinang, Maludam, Miri, and Kuching inSarawak, Malaysia.Five fecessamples were collected randomly from
each site. One gram of the fecessample was diluted in 9 mLof 0.85% normalsaline solution. The diluted sample
was plated on TrypticaseSoy agar plates and incubated at 37±1 °C for 24 h. A total of 500 bacteria isolates were
then identified using 16S rRNA analysis method. Disc diffusion method was then used to confirm the resistant
phenotypes of these isolates. The results showed that the means of the bacterial colony count were significantly
-1 different (p<0.05) from one another, with the highestlog CFU g (9.22±0.72) found in KotaSamarahan and the 10 -1 lowest log CFU g (6.03±0.62) in Betong. Besides, the isolated bacteria were identified as 96% Gram positive 10 bacteria and 4% Gram negative bacteria. The isolated bacteria were highly resistant to penicillin G (36.80±23.87%), ampicillin (28.60±17.13%), and rifampicin (16.90±13.70%). Thus, swiftlet feces are good
reservoirfor a range of antibiotic resistant bacteria whichmay pose a potential health hazard to human. |
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