The functional -94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer.
Objective: To investigate the allele and genotype frequencies of NFKBI -94 ins/de! ATTG (rs28720239) polymorphism and to evaluate the association between the polymorphism and colorectal cancer (CRC) risk in Malaysian population. Methods: Genomic DNA was extracted from the peripheral blood samples of...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | en |
| Published: |
Elsevier Science, Ltd.
2013
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/30234/1/The%20functional%20.pdf http://ir.unimas.my/id/eprint/30234/ https://www.sciencedirect.com/science/article/abs/pii/S1877782113000945 https://doi.org/10.1016/j.canep.2013.05.007 |
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| Summary: | Objective: To investigate the allele and genotype frequencies of NFKBI -94 ins/de! ATTG (rs28720239) polymorphism and to evaluate the association between the polymorphism and colorectal cancer (CRC) risk in Malaysian population. Methods: Genomic DNA was extracted from the peripheral blood samples of 474 study subjects, which consisted of 237 histopathologically confirmed CRC patients and an equal number of cancer-free controls. The NFKBI -94 ins/de! ATTG (rs28720239) polymorphism was genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and confirmed by DNA sequencing. The association between the polymorphic genotypes and CRC risk was evaluated by deriving odds ratios (ORs) and 95% confidence intervals (Cls) using unconditional logistic regression analysis. Results: The frequencies of wildtype (del/del), heterozygous ( del/ins) and variant (ins/ins) genotypes in CRC patients were 31.7%, 53.6% and 14.8%, respectively, while those in cancer-free controls were 35.0%, 58.2% and 6.8%, respectively. The frequency of the variant genotype was significantly higher in cases compared to controls (P < 0.01 ). Evaluation of the risk association of the polymorphic genotypes revealed that the variant genotype could contribute to a significantly increased risk of CRC (OR= 2.42, 95% CI= 1.24-4.73, P < 0.01 ). Conclusions: The variant allele of NFKBI -94 ins/de! ATTG (rs28362491) polymorphism is associated with higher risk of sporadic CRC in Malaysian population. |
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