Rapid in vitro Propagation and Efficient Acclimatisation Protocols of Neolamarckia cadamba
Background and Objective: Neolamarckia cadamba is a fast-growing commercial timber tree species with considerable economic returns to the growers on a rotation period of 4-10 years. The present study was aimed to establish an efficient micropropagation protocol for N. cadamba through direct organoge...
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Asian Network for Scientific Information
2019
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| Online Access: | http://ir.unimas.my/id/eprint/28310/1/Ho%20Wei-Seng.pdf http://ir.unimas.my/id/eprint/28310/ https://scialert.net/current.php?issn=1682-3974 |
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| author | Ho, Wei Seng Mok, Pei Kieng |
| author_facet | Ho, Wei Seng Mok, Pei Kieng |
| author_sort | Ho, Wei Seng |
| building | Centre for Academic Information Services (CAIS) |
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| content_provider | Universiti Malaysia Sarawak |
| content_source | UNIMAS Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Background and Objective: Neolamarckia cadamba is a fast-growing commercial timber tree species with considerable economic returns to the growers on a rotation period of 4-10 years. The present study was aimed to establish an efficient micropropagation protocol for N. cadamba through direct organogenesis by using nodal explants. Materials and Methods: The nodal explants from in vitro-germinated seedlings were cultured on B5 medium supplemented with various BAP concentrations. The in vitro shoots were then rooted in 1/2 B5 medium added with growth regulators such as IBA, NAA and PBZ. Three types of potting media were tested for transplantation efficiency.
Results: The B5 medium supplemented with 1.0 mg LG1 BAP provided the most suitable medium for shoot induction from nodal explants with a mean of 5.4 shoots per explant. The subculture interval could be shortened by proliferating the regenerated axillary shoots on B5
medium supplemented with 0.8 mg LG1 BAP. Half-strength B5 medium enriched with 0.1 mg LG1 PBZ was able to induce root growth with 100% of root formation and resulted in more than 95% survival during acclimatisation stage. Conclusion: This micropropagation protocol could pave the way for mass production of quality N. cadamba seedlings for industrial tree plantation development in order to assure the local timber industries to meet the global demand for wood. Therefore, this could reduce the reliance on natural forests for wood production. |
| format | Article |
| id | my.unimas.ir-28310 |
| institution | Universiti Malaysia Sarawak |
| language | en |
| publishDate | 2019 |
| publisher | Asian Network for Scientific Information |
| record_format | eprints |
| spelling | my.unimas.ir-283102021-03-30T08:00:14Z http://ir.unimas.my/id/eprint/28310/ Rapid in vitro Propagation and Efficient Acclimatisation Protocols of Neolamarckia cadamba Ho, Wei Seng Mok, Pei Kieng SB Plant culture SD Forestry Background and Objective: Neolamarckia cadamba is a fast-growing commercial timber tree species with considerable economic returns to the growers on a rotation period of 4-10 years. The present study was aimed to establish an efficient micropropagation protocol for N. cadamba through direct organogenesis by using nodal explants. Materials and Methods: The nodal explants from in vitro-germinated seedlings were cultured on B5 medium supplemented with various BAP concentrations. The in vitro shoots were then rooted in 1/2 B5 medium added with growth regulators such as IBA, NAA and PBZ. Three types of potting media were tested for transplantation efficiency. Results: The B5 medium supplemented with 1.0 mg LG1 BAP provided the most suitable medium for shoot induction from nodal explants with a mean of 5.4 shoots per explant. The subculture interval could be shortened by proliferating the regenerated axillary shoots on B5 medium supplemented with 0.8 mg LG1 BAP. Half-strength B5 medium enriched with 0.1 mg LG1 PBZ was able to induce root growth with 100% of root formation and resulted in more than 95% survival during acclimatisation stage. Conclusion: This micropropagation protocol could pave the way for mass production of quality N. cadamba seedlings for industrial tree plantation development in order to assure the local timber industries to meet the global demand for wood. Therefore, this could reduce the reliance on natural forests for wood production. Asian Network for Scientific Information 2019-11 Article PeerReviewed text en http://ir.unimas.my/id/eprint/28310/1/Ho%20Wei-Seng.pdf Ho, Wei Seng and Mok, Pei Kieng (2019) Rapid in vitro Propagation and Efficient Acclimatisation Protocols of Neolamarckia cadamba. Asian Journal of Plant Sciences. pp. 1-11. ISSN 1682-3974 https://scialert.net/current.php?issn=1682-3974 |
| spellingShingle | SB Plant culture SD Forestry Ho, Wei Seng Mok, Pei Kieng Rapid in vitro Propagation and Efficient Acclimatisation Protocols of Neolamarckia cadamba |
| title | Rapid in vitro Propagation and Efficient Acclimatisation Protocols of Neolamarckia cadamba |
| title_full | Rapid in vitro Propagation and Efficient Acclimatisation Protocols of Neolamarckia cadamba |
| title_fullStr | Rapid in vitro Propagation and Efficient Acclimatisation Protocols of Neolamarckia cadamba |
| title_full_unstemmed | Rapid in vitro Propagation and Efficient Acclimatisation Protocols of Neolamarckia cadamba |
| title_short | Rapid in vitro Propagation and Efficient Acclimatisation Protocols of Neolamarckia cadamba |
| title_sort | rapid in vitro propagation and efficient acclimatisation protocols of neolamarckia cadamba |
| topic | SB Plant culture SD Forestry |
| url | http://ir.unimas.my/id/eprint/28310/1/Ho%20Wei-Seng.pdf http://ir.unimas.my/id/eprint/28310/ https://scialert.net/current.php?issn=1682-3974 |
| url_provider | http://ir.unimas.my/ |