Phytochemical, cytotoxicity and antioxidant activities of the stem bark of Piper arborescens
Crude extract from stem bark of P. arborescens was fractionated by using column chromatography to isolate and purify its metabolite content. Six secondary metabolites were successfully isolated and their identification was performed by using Gas Chromatography - Mass Spectrometry (GC-MS), Nuclear...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | en |
| Published: |
Penerbit UTM
2017
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/19606/1/706-3473-1-PB%20%28abstrak%29.pdf http://ir.unimas.my/id/eprint/19606/ https://mjfas.utm.my/ |
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| Summary: | Crude extract from stem bark of P. arborescens was fractionated by using column chromatography to
isolate and purify its metabolite content. Six secondary metabolites were successfully isolated and
their identification was performed by using Gas Chromatography - Mass Spectrometry (GC-MS),
Nuclear Magnetic Resonance (NMR) and Fourier Transform Infrared (FTIR) spectoscopy. The isolated
metabolites were identified as caryophyllene oxide (1), α-bisabolol (2), benzamide 2-(methylamino)
(3), 2-ethylpiperidine (4), piperine (5) and methyl eugenol (6). Toxicity test on the four crude extracts
of P. arborescens shows high cytotoxicity against Artemia salina brine shrimp with LC50 values ranging
from 13.12 to 58.70 µg/mL. Greater cytotoxicity of the crude extracts of P. arborescens indicated the
presence of potent cytotoxic components in this Piper spp. Antioxidant assay of P. arborescens against
2-diphenyl-1-picrylhydrazyl (DPPH) indicated moderate antioxidant activities of methanol,
dichloromethane, chloroform and hexane crude extracts with EC50 values of 21.68, 23.82, 32.88 and
36.88 µg/mL, respectively. It is suggested that the six secondary metabolites identified in P.
arborescens contribute as an active content for the cytotoxicity and antioxidant activities. This study
showed that the crude extracts of P. arborescens is definitely having potential to be used as a source
of natural product of various application |
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