Efficient and precise engineering of a 200 kb b-globin human/bacterial artificial chromosome in E. coli DH10B using an inducible homologous recombination system
Gene therapy studies require techniques that allow alter- adapted an inducible homologous recombination system ation of human genomic DNA sequences. Bacterial arti- for use in E. coli DH10B cells, the host strain for BACs ficial chromosome cloning systems (BACs/PACs) bridge and PACs. Using this sy...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | en |
| Published: |
Stockton Press
1999
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/16746/1/Efficient.pdf http://ir.unimas.my/id/eprint/16746/ http://www.nature.com/gt/journal/v6/n3/full/3300901a.html |
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