Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants
This study characterised 7 isolates of Edwardsiella tarda from African catfish, Clarias gariepinus. The isolated E. tarda were identified using a combination of 29 conventional tests and commercial kit. Random amplification of polymorphic DNApolymerase chain reaction (RAPD PCR) analysis was done o...
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| Format: | Thesis |
| Language: | en |
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Fakulti Agroteknologi dan Sains Makanan
2011
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| Online Access: | http://hdl.handle.net/123456789/802 |
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| _version_ | 1832418872204984320 |
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| author | Lee Seong Wei |
| author_facet | Lee Seong Wei |
| author_sort | Lee Seong Wei |
| building | Perpustakaan Sultanah Nur Zahirah |
| collection | Institutional Repository |
| content_provider | Universiti Malaysia Terengganu |
| content_source | UMT-IR |
| continent | Asia |
| country | Malaysia |
| description | This study characterised 7 isolates of Edwardsiella tarda from African catfish,
Clarias gariepinus. The isolated E. tarda were identified using a combination of 29
conventional tests and commercial kit. Random amplification of polymorphic DNApolymerase chain reaction (RAPD PCR) analysis was done on these bacterial isolates
using three universal primers, namely WTP, (GTG)s and M13 universal. Characterisation of whole cell protein profiles of the isolates were also carried out using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). In the present study, minimum inhibitory concentration (MIC) values of 20 edible plant extracts against E. tarda were determined through a two fold microdilution technique. |
| format | Thesis |
| id | my.umt.ir-802 |
| institution | Universiti Malaysia Terengganu |
| language | en |
| publishDate | 2011 |
| publisher | Fakulti Agroteknologi dan Sains Makanan |
| record_format | dspace |
| spelling | my.umt.ir-8022016-03-11T20:40:36Z Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants Lee Seong Wei QR 82 .E6 L4 2009 Lee Seong Wei Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants Edwardsiella tarda This study characterised 7 isolates of Edwardsiella tarda from African catfish, Clarias gariepinus. The isolated E. tarda were identified using a combination of 29 conventional tests and commercial kit. Random amplification of polymorphic DNApolymerase chain reaction (RAPD PCR) analysis was done on these bacterial isolates using three universal primers, namely WTP, (GTG)s and M13 universal. Characterisation of whole cell protein profiles of the isolates were also carried out using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). In the present study, minimum inhibitory concentration (MIC) values of 20 edible plant extracts against E. tarda were determined through a two fold microdilution technique. 2011-07-12T06:39:03Z 2011-07-12T06:39:03Z 2009-05 Thesis http://hdl.handle.net/123456789/802 en QR 82 .E6 L4 2009; application/pdf application/pdf Fakulti Agroteknologi dan Sains Makanan |
| spellingShingle | QR 82 .E6 L4 2009 Lee Seong Wei Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants Edwardsiella tarda Lee Seong Wei Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants |
| title | Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants |
| title_full | Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants |
| title_fullStr | Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants |
| title_full_unstemmed | Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants |
| title_short | Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants |
| title_sort | characteristation of edwardsiella tarda isolated from local calrias gariepinus and potential remedies from edible plants |
| topic | QR 82 .E6 L4 2009 Lee Seong Wei Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants Edwardsiella tarda |
| url | http://hdl.handle.net/123456789/802 |
| url_provider | http://umt-ir.umt.edu.my:8080/ |