Whole-genome sequencing of Proteus mirabilis strain CM018 isolated from retail chicken meat
In this study, Proteus mirabilis strain CM018 was isolated from retail chicken meat and confirmed by 16S rRNA gene sequencing. Whole-genome sequencing (WGS) was performed using a combination of Illumina and Oxford Nanopore Technologies (ONT), followed by hybrid assembly and polishing tools. The geno...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | en |
| Published: |
Rynnye Lyan Resources
2025
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| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/45689/1/FULLTEXT.pdf https://eprints.ums.edu.my/id/eprint/45689/ http:// doi.org/10.26656/lifr.1.e25039 |
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| Summary: | In this study, Proteus mirabilis strain CM018 was isolated from retail chicken meat and confirmed by 16S rRNA gene sequencing. Whole-genome sequencing (WGS) was performed using a combination of Illumina and Oxford Nanopore Technologies (ONT), followed by hybrid assembly and polishing tools. The genome has 3,812,096 bp and 3342 protein coding sequences with GC content of 38.6%. Antibiotic resistance genes (ARGs) such as Tet(J), Cata, crp, and 3 CRISPR arrays were found in the genome. Additionally, antiviral defense systems of Gabija, PARIS, PD-Lamda-5 and Septu were detected using the webserver PADLOC and Defense Finder. Further, experimental results showed that a siphoviridae prophage was isolated using mitomycin C induction. The findings contribute to the understanding of P. mirabilis in retail chicken meat and its potential to pose a substantial public health threat. |
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