Staphylococcus aureus lytic bacteriophage: Isolation and application evaluation
Staphylococcus aureus is a signifcant pathogen associated with various illnesses, including food poisoning. The development of efective treatments is challenging, necessitating the exploration of novel antimicrobial options. Bacteriophages (phages) have emerged as promising candidates in this regard...
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Springer
2024
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| Online Access: | https://eprints.ums.edu.my/id/eprint/44314/1/FULLTEXT.pdf https://eprints.ums.edu.my/id/eprint/44314/ https://doi.org/10.1007/s00003-024-01479-8 |
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| author | Fatmawati Lambuka Nurzafrah Mazlan Dalene Lesen Elexson Nillian Tze, Young Thung |
| author_facet | Fatmawati Lambuka Nurzafrah Mazlan Dalene Lesen Elexson Nillian Tze, Young Thung |
| author_sort | Fatmawati Lambuka |
| building | UMS Library |
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| content_provider | Universiti Malaysia Sabah |
| content_source | UMS Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Staphylococcus aureus is a signifcant pathogen associated with various illnesses, including food poisoning. The development of efective treatments is challenging, necessitating the exploration of novel antimicrobial options. Bacteriophages (phages) have emerged as promising candidates in this regard. In this study, a virulent phage called mSA4 was isolated and characterized. Furthermore, its efcacy in combating S. aureus bioflms and growth in various food products was evaluated. Phage mSA4 demonstrated a broad host range, targeting both S. aureus and methicillin-resistant S. aureus strains. Belonging to the Myoviridae family, it exhibited rapid adsorption (over 50% in 3 min), a short latent period (20 min), and a burst size of 97 phage particles per infected bacterial cell. Furthermore, phage mSA4 displayed stability across a wide range of pH values and temperatures, and efectively degraded established bioflms. Its performance was evaluated in chocolate milk, beef meat, and iceberg lettuce, resulting in signifcant reductions in bacterial counts (2.1 log CFU/mL, 2.8 log CFU/cm2 , and 3.2 log CFU/cm2 , respectively). These fndings underscore the potential of phage mSA4 as a natural biocontrol agent against S. aureus. |
| format | Article |
| id | my.ums.eprints-44314 |
| institution | Universiti Malaysia Sabah |
| language | en |
| publishDate | 2024 |
| publisher | Springer |
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| spelling | my.ums.eprints-443142025-07-21T07:16:33Z https://eprints.ums.edu.my/id/eprint/44314/ Staphylococcus aureus lytic bacteriophage: Isolation and application evaluation Fatmawati Lambuka Nurzafrah Mazlan Dalene Lesen Elexson Nillian Tze, Young Thung QR75-99.5 Bacteria RC109-216 Infectious and parasitic diseases Staphylococcus aureus is a signifcant pathogen associated with various illnesses, including food poisoning. The development of efective treatments is challenging, necessitating the exploration of novel antimicrobial options. Bacteriophages (phages) have emerged as promising candidates in this regard. In this study, a virulent phage called mSA4 was isolated and characterized. Furthermore, its efcacy in combating S. aureus bioflms and growth in various food products was evaluated. Phage mSA4 demonstrated a broad host range, targeting both S. aureus and methicillin-resistant S. aureus strains. Belonging to the Myoviridae family, it exhibited rapid adsorption (over 50% in 3 min), a short latent period (20 min), and a burst size of 97 phage particles per infected bacterial cell. Furthermore, phage mSA4 displayed stability across a wide range of pH values and temperatures, and efectively degraded established bioflms. Its performance was evaluated in chocolate milk, beef meat, and iceberg lettuce, resulting in signifcant reductions in bacterial counts (2.1 log CFU/mL, 2.8 log CFU/cm2 , and 3.2 log CFU/cm2 , respectively). These fndings underscore the potential of phage mSA4 as a natural biocontrol agent against S. aureus. Springer 2024-02-15 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/44314/1/FULLTEXT.pdf Fatmawati Lambuka and Nurzafrah Mazlan and Dalene Lesen and Elexson Nillian and Tze, Young Thung (2024) Staphylococcus aureus lytic bacteriophage: Isolation and application evaluation. Journal of Consumer Protection and Food Safety, 19. pp. 235-243. ISSN 1661-5751 https://doi.org/10.1007/s00003-024-01479-8 |
| spellingShingle | QR75-99.5 Bacteria RC109-216 Infectious and parasitic diseases Fatmawati Lambuka Nurzafrah Mazlan Dalene Lesen Elexson Nillian Tze, Young Thung Staphylococcus aureus lytic bacteriophage: Isolation and application evaluation |
| title | Staphylococcus aureus lytic bacteriophage: Isolation and application evaluation |
| title_full | Staphylococcus aureus lytic bacteriophage: Isolation and application evaluation |
| title_fullStr | Staphylococcus aureus lytic bacteriophage: Isolation and application evaluation |
| title_full_unstemmed | Staphylococcus aureus lytic bacteriophage: Isolation and application evaluation |
| title_short | Staphylococcus aureus lytic bacteriophage: Isolation and application evaluation |
| title_sort | staphylococcus aureus lytic bacteriophage: isolation and application evaluation |
| topic | QR75-99.5 Bacteria RC109-216 Infectious and parasitic diseases |
| url | https://eprints.ums.edu.my/id/eprint/44314/1/FULLTEXT.pdf https://eprints.ums.edu.my/id/eprint/44314/ https://doi.org/10.1007/s00003-024-01479-8 |
| url_provider | http://eprints.ums.edu.my/ |