Subcritical water extraction on phenolic, flavonoid and antioxidant activity from orthosiphon stamineus leaves: experimental and optimization

Orthosiphon stamineus is rich in phenolic and flavonoid compounds, contributing to its antioxidant properties. This study focuses optimizes the extraction of bioactive compounds from Orthosiphon stamineus leaves using subcritical water (SCW) extraction. Response surface methodology was employed, wit...

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Main Authors: Sarah Aisyah Khurun Hizar, Nicky Rahmana Putra, Rovina Kobun, Siti Faridah Mohd Amin, Jumardi Roslan, Macdalyna Esther Ronie, Muhammad Abbas Ahmad Zaini, Hasmadi Mamat, Ahmad Hazim Abdul Aziz
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Language:en
en
Published: Kuwait University 2024
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Online Access:https://eprints.ums.edu.my/id/eprint/41973/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/41973/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/41973/
https://doi.org/10.1016/j.jer.2024.10.001
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author Sarah Aisyah Khurun Hizar
Nicky Rahmana Putra
Rovina Kobun
Siti Faridah Mohd Amin
Jumardi Roslan
Macdalyna Esther Ronie
Muhammad Abbas Ahmad Zaini
Hasmadi Mamat
Ahmad Hazim Abdul Aziz
author_facet Sarah Aisyah Khurun Hizar
Nicky Rahmana Putra
Rovina Kobun
Siti Faridah Mohd Amin
Jumardi Roslan
Macdalyna Esther Ronie
Muhammad Abbas Ahmad Zaini
Hasmadi Mamat
Ahmad Hazim Abdul Aziz
author_sort Sarah Aisyah Khurun Hizar
building UMS Library
collection Institutional Repository
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
continent Asia
country Malaysia
description Orthosiphon stamineus is rich in phenolic and flavonoid compounds, contributing to its antioxidant properties. This study focuses optimizes the extraction of bioactive compounds from Orthosiphon stamineus leaves using subcritical water (SCW) extraction. Response surface methodology was employed, with temperature (120–180 °C), flow rate (2–8 mL min−1), and extraction time (5–15 min) as independent variables. Dependent variables included extract yield, total phenolic compounds (TPC), total flavonoid compounds (TFC), and antioxidant activity (AA). Optimal conditions were found at 148 °C, 5.1 mL min−1, and 10.5 min, resulting in a maximum yield of 317.6 mg g−1, TPC of 12.71 mg 100 g−1, TFC of 74.09 mg 100 g−1, and AA of 87.48 %. The research highlights the significant influence of temperature as the most crucial parameter, followed by flow rate and extraction time, in achieving the highest yield and bioactive compound content. This investigation holds potential implications for the food processing industry, showcasing the relevance of optimizing extraction conditions to enhance the nutritional and functional properties of natural ingredients like Orthosiphon stamineus in food products.
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spelling my.ums.eprints-419732024-11-20T04:32:40Z https://eprints.ums.edu.my/id/eprint/41973/ Subcritical water extraction on phenolic, flavonoid and antioxidant activity from orthosiphon stamineus leaves: experimental and optimization Sarah Aisyah Khurun Hizar Nicky Rahmana Putra Rovina Kobun Siti Faridah Mohd Amin Jumardi Roslan Macdalyna Esther Ronie Muhammad Abbas Ahmad Zaini Hasmadi Mamat Ahmad Hazim Abdul Aziz Q1-390 Science (General) QK710-899 Plant physiology Orthosiphon stamineus is rich in phenolic and flavonoid compounds, contributing to its antioxidant properties. This study focuses optimizes the extraction of bioactive compounds from Orthosiphon stamineus leaves using subcritical water (SCW) extraction. Response surface methodology was employed, with temperature (120–180 °C), flow rate (2–8 mL min−1), and extraction time (5–15 min) as independent variables. Dependent variables included extract yield, total phenolic compounds (TPC), total flavonoid compounds (TFC), and antioxidant activity (AA). Optimal conditions were found at 148 °C, 5.1 mL min−1, and 10.5 min, resulting in a maximum yield of 317.6 mg g−1, TPC of 12.71 mg 100 g−1, TFC of 74.09 mg 100 g−1, and AA of 87.48 %. The research highlights the significant influence of temperature as the most crucial parameter, followed by flow rate and extraction time, in achieving the highest yield and bioactive compound content. This investigation holds potential implications for the food processing industry, showcasing the relevance of optimizing extraction conditions to enhance the nutritional and functional properties of natural ingredients like Orthosiphon stamineus in food products. Kuwait University 2024 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/41973/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/41973/2/FULL%20TEXT.pdf Sarah Aisyah Khurun Hizar and Nicky Rahmana Putra and Rovina Kobun and Siti Faridah Mohd Amin and Jumardi Roslan and Macdalyna Esther Ronie and Muhammad Abbas Ahmad Zaini and Hasmadi Mamat and Ahmad Hazim Abdul Aziz (2024) Subcritical water extraction on phenolic, flavonoid and antioxidant activity from orthosiphon stamineus leaves: experimental and optimization. Journal of Engineering Research. pp. 1-8. ISSN 2307-1877 https://doi.org/10.1016/j.jer.2024.10.001
spellingShingle Q1-390 Science (General)
QK710-899 Plant physiology
Sarah Aisyah Khurun Hizar
Nicky Rahmana Putra
Rovina Kobun
Siti Faridah Mohd Amin
Jumardi Roslan
Macdalyna Esther Ronie
Muhammad Abbas Ahmad Zaini
Hasmadi Mamat
Ahmad Hazim Abdul Aziz
Subcritical water extraction on phenolic, flavonoid and antioxidant activity from orthosiphon stamineus leaves: experimental and optimization
title Subcritical water extraction on phenolic, flavonoid and antioxidant activity from orthosiphon stamineus leaves: experimental and optimization
title_full Subcritical water extraction on phenolic, flavonoid and antioxidant activity from orthosiphon stamineus leaves: experimental and optimization
title_fullStr Subcritical water extraction on phenolic, flavonoid and antioxidant activity from orthosiphon stamineus leaves: experimental and optimization
title_full_unstemmed Subcritical water extraction on phenolic, flavonoid and antioxidant activity from orthosiphon stamineus leaves: experimental and optimization
title_short Subcritical water extraction on phenolic, flavonoid and antioxidant activity from orthosiphon stamineus leaves: experimental and optimization
title_sort subcritical water extraction on phenolic, flavonoid and antioxidant activity from orthosiphon stamineus leaves: experimental and optimization
topic Q1-390 Science (General)
QK710-899 Plant physiology
url https://eprints.ums.edu.my/id/eprint/41973/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/41973/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/41973/
https://doi.org/10.1016/j.jer.2024.10.001
url_provider http://eprints.ums.edu.my/