The effect of simulated copper stress on genes associated with the metabolism of polyphosphates and polyhydroxyalkanoates in Bacillus thuringiensis Y1
Aims: Bacillus thuringiensis Y1 is a copper-tolerant bacterium that can serve as a model for the elucidation of the mechanism of energy metabolism under simulated copper stress. This study aimed to elucidate the effects of simulated copper stress on the genes associated with the biosynthesis of poly...
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| Language: | en en |
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Malaysian Society for Microbiology
2023
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| Online Access: | https://eprints.ums.edu.my/id/eprint/38821/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/38821/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/38821/ http://dx.doi.org/10.21161/mjm.230003 |
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| author | Low Yi Yik Grace Joy Wei Lie Chin Collin Glen Joseph Kenneth Francis Rodrigues |
| author_facet | Low Yi Yik Grace Joy Wei Lie Chin Collin Glen Joseph Kenneth Francis Rodrigues |
| author_sort | Low Yi Yik |
| building | UMS Library |
| collection | Institutional Repository |
| content_provider | Universiti Malaysia Sabah |
| content_source | UMS Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Aims: Bacillus thuringiensis Y1 is a copper-tolerant bacterium that can serve as a model for the elucidation of the mechanism of energy metabolism under simulated copper stress. This study aimed to elucidate the effects of simulated copper stress on the genes associated with the biosynthesis of polyhydroxyalkanoates (PAH) and the metabolism of polyphosphates (PP). Methodology and results: The gene expression study involved the growth of the bacterium in nutrient broth supplemented with two concentrations of copper sulphate (0.4 mM and 0.8 mM), followed by RNA extraction and quantification of four and 11 genes associated with the metabolism of polyphosphate (PP) and polyhydroxyalkanoates (PAHs) respectively, using reverse transcription quantitative polymerase chain reaction (RT-qPCR). Three genes associated with polyphosphate metabolism, which are polyphosphate kinase (ppk), exopolyphosphatase (ppx) and NAD kinase (ppnk), were all shown to be upregulated by both 0.4 mM and 0.8 mM copper, except for the 5’-nucleotidase (surE) gene that was downregulated under the second treatment. Among the 11 genes associated with the metabolism of polyhydroxyalkanoates, only the 3-ketoacyl-CoA-thiolase (phaA) gene was upregulated in both treatments and highly expressed in the second treatment; the majority were downregulated and repressed. Conclusion, significance and impact of study: The study demonstrated that copper induces the metabolism of polyphosphates in B. thuringiensis Y1 that serve as an alternative source of energy under copper stress. This model can be extended to the study of other species of Bacillus under environmental stress. |
| format | Article |
| id | my.ums.eprints-38821 |
| institution | Universiti Malaysia Sabah |
| language | en en |
| publishDate | 2023 |
| publisher | Malaysian Society for Microbiology |
| record_format | eprints |
| spelling | my.ums.eprints-388212024-06-12T02:01:44Z https://eprints.ums.edu.my/id/eprint/38821/ The effect of simulated copper stress on genes associated with the metabolism of polyphosphates and polyhydroxyalkanoates in Bacillus thuringiensis Y1 Low Yi Yik Grace Joy Wei Lie Chin Collin Glen Joseph Kenneth Francis Rodrigues QP501-801 Animal biochemistry QR75-99.5 Bacteria Aims: Bacillus thuringiensis Y1 is a copper-tolerant bacterium that can serve as a model for the elucidation of the mechanism of energy metabolism under simulated copper stress. This study aimed to elucidate the effects of simulated copper stress on the genes associated with the biosynthesis of polyhydroxyalkanoates (PAH) and the metabolism of polyphosphates (PP). Methodology and results: The gene expression study involved the growth of the bacterium in nutrient broth supplemented with two concentrations of copper sulphate (0.4 mM and 0.8 mM), followed by RNA extraction and quantification of four and 11 genes associated with the metabolism of polyphosphate (PP) and polyhydroxyalkanoates (PAHs) respectively, using reverse transcription quantitative polymerase chain reaction (RT-qPCR). Three genes associated with polyphosphate metabolism, which are polyphosphate kinase (ppk), exopolyphosphatase (ppx) and NAD kinase (ppnk), were all shown to be upregulated by both 0.4 mM and 0.8 mM copper, except for the 5’-nucleotidase (surE) gene that was downregulated under the second treatment. Among the 11 genes associated with the metabolism of polyhydroxyalkanoates, only the 3-ketoacyl-CoA-thiolase (phaA) gene was upregulated in both treatments and highly expressed in the second treatment; the majority were downregulated and repressed. Conclusion, significance and impact of study: The study demonstrated that copper induces the metabolism of polyphosphates in B. thuringiensis Y1 that serve as an alternative source of energy under copper stress. This model can be extended to the study of other species of Bacillus under environmental stress. Malaysian Society for Microbiology 2023 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/38821/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/38821/2/FULL%20TEXT.pdf Low Yi Yik and Grace Joy Wei Lie Chin and Collin Glen Joseph and Kenneth Francis Rodrigues (2023) The effect of simulated copper stress on genes associated with the metabolism of polyphosphates and polyhydroxyalkanoates in Bacillus thuringiensis Y1. Malaysian Journal of Microbiology, 19 (6). pp. 1-9. ISSN 2231-7538 http://dx.doi.org/10.21161/mjm.230003 |
| spellingShingle | QP501-801 Animal biochemistry QR75-99.5 Bacteria Low Yi Yik Grace Joy Wei Lie Chin Collin Glen Joseph Kenneth Francis Rodrigues The effect of simulated copper stress on genes associated with the metabolism of polyphosphates and polyhydroxyalkanoates in Bacillus thuringiensis Y1 |
| title | The effect of simulated copper stress on genes associated with the metabolism of polyphosphates and polyhydroxyalkanoates in Bacillus thuringiensis Y1 |
| title_full | The effect of simulated copper stress on genes associated with the metabolism of polyphosphates and polyhydroxyalkanoates in Bacillus thuringiensis Y1 |
| title_fullStr | The effect of simulated copper stress on genes associated with the metabolism of polyphosphates and polyhydroxyalkanoates in Bacillus thuringiensis Y1 |
| title_full_unstemmed | The effect of simulated copper stress on genes associated with the metabolism of polyphosphates and polyhydroxyalkanoates in Bacillus thuringiensis Y1 |
| title_short | The effect of simulated copper stress on genes associated with the metabolism of polyphosphates and polyhydroxyalkanoates in Bacillus thuringiensis Y1 |
| title_sort | effect of simulated copper stress on genes associated with the metabolism of polyphosphates and polyhydroxyalkanoates in bacillus thuringiensis y1 |
| topic | QP501-801 Animal biochemistry QR75-99.5 Bacteria |
| url | https://eprints.ums.edu.my/id/eprint/38821/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/38821/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/38821/ http://dx.doi.org/10.21161/mjm.230003 |
| url_provider | http://eprints.ums.edu.my/ |