Antioxidants Discovery for Differentiation of Monofloral Stingless Bee Honeys Using Ambient Mass Spectrometry and Metabolomics Approaches education
Stingless bee honey (SBH) is a natural, sweet product produced by stingless bees (Meliponini tribe) that has been used as a traditional medicine to treat various illnesses. It has been shown that SBH has high nutritional value and health-promoting properties due to the presence of plant bioactive co...
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Multidisciplinary Digital Publishing Institute (MDPI)
2023
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| Online Access: | https://eprints.ums.edu.my/id/eprint/38310/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/38310/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/38310/ https://doi.org/10.3390/foods12122404 |
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| author | Wei, Chean Chuah Huei, Hong Lee Daniel H. J. Ng Ai, Ling Ho Mohd Rosni Sulaiman Fook, Yee Chye |
| author_facet | Wei, Chean Chuah Huei, Hong Lee Daniel H. J. Ng Ai, Ling Ho Mohd Rosni Sulaiman Fook, Yee Chye |
| author_sort | Wei, Chean Chuah |
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| content_provider | Universiti Malaysia Sabah |
| content_source | UMS Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Stingless bee honey (SBH) is a natural, sweet product produced by stingless bees (Meliponini tribe) that has been used as a traditional medicine to treat various illnesses. It has been shown that SBH has high nutritional value and health-promoting properties due to the presence of plant bioactive compounds from different botanical flora of the foraged nectar. In this study, the antioxidant activities of seven monofloral honeys from acacia, agarwood, coconut, dwarf mountain pine (DMP), Mexican creeper (MC), rubber, and starfruit botanical origins were investigated. The antioxidant properties of SBH studied had a range from 19.7 to 31.4 mM TE/mg for DPPH assays, 16.1 to 29.9 mM TE/mg for ABTS assays, 69.0 to 167.6 mM TE/mg for ORAC assays, and 45.5 to 89.3 mM Fe2+/mg for FRAP assays. Acacia honey showed the highest level of antioxidant properties. The models built from mass spectral fingerprints from direct ambient mass spectrometry showed distinct clusters of SBH by botanical origin and correlated with the antioxidant properties. An untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomics approach was undertaken to identify the antioxidant compounds that could explain the unique antioxidant and compositional profiles of the monofloral SBH by its botanical origin. The antioxidants that were identified predominantly consisted of alkaloids and flavonoids. Flavonoid derivatives, which are potent antioxidants, were found to be key markers of acacia honey. This work provides the fundamental basis for the identification of potential antioxidant markers in SBH associated with the botanical origin of the foraged nectar. |
| format | Article |
| id | my.ums.eprints-38310 |
| institution | Universiti Malaysia Sabah |
| language | en en |
| publishDate | 2023 |
| publisher | Multidisciplinary Digital Publishing Institute (MDPI) |
| record_format | eprints |
| spelling | my.ums.eprints-383102024-02-22T03:02:40Z https://eprints.ums.edu.my/id/eprint/38310/ Antioxidants Discovery for Differentiation of Monofloral Stingless Bee Honeys Using Ambient Mass Spectrometry and Metabolomics Approaches education Wei, Chean Chuah Huei, Hong Lee Daniel H. J. Ng Ai, Ling Ho Mohd Rosni Sulaiman Fook, Yee Chye QP501-801 Animal biochemistry SF521-539.8 Bee culture Stingless bee honey (SBH) is a natural, sweet product produced by stingless bees (Meliponini tribe) that has been used as a traditional medicine to treat various illnesses. It has been shown that SBH has high nutritional value and health-promoting properties due to the presence of plant bioactive compounds from different botanical flora of the foraged nectar. In this study, the antioxidant activities of seven monofloral honeys from acacia, agarwood, coconut, dwarf mountain pine (DMP), Mexican creeper (MC), rubber, and starfruit botanical origins were investigated. The antioxidant properties of SBH studied had a range from 19.7 to 31.4 mM TE/mg for DPPH assays, 16.1 to 29.9 mM TE/mg for ABTS assays, 69.0 to 167.6 mM TE/mg for ORAC assays, and 45.5 to 89.3 mM Fe2+/mg for FRAP assays. Acacia honey showed the highest level of antioxidant properties. The models built from mass spectral fingerprints from direct ambient mass spectrometry showed distinct clusters of SBH by botanical origin and correlated with the antioxidant properties. An untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomics approach was undertaken to identify the antioxidant compounds that could explain the unique antioxidant and compositional profiles of the monofloral SBH by its botanical origin. The antioxidants that were identified predominantly consisted of alkaloids and flavonoids. Flavonoid derivatives, which are potent antioxidants, were found to be key markers of acacia honey. This work provides the fundamental basis for the identification of potential antioxidant markers in SBH associated with the botanical origin of the foraged nectar. Multidisciplinary Digital Publishing Institute (MDPI) 2023 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/38310/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/38310/2/FULL%20TEXT.pdf Wei, Chean Chuah and Huei, Hong Lee and Daniel H. J. Ng and Ai, Ling Ho and Mohd Rosni Sulaiman and Fook, Yee Chye (2023) Antioxidants Discovery for Differentiation of Monofloral Stingless Bee Honeys Using Ambient Mass Spectrometry and Metabolomics Approaches education. Foods, 12. pp. 1-12. https://doi.org/10.3390/foods12122404 |
| spellingShingle | QP501-801 Animal biochemistry SF521-539.8 Bee culture Wei, Chean Chuah Huei, Hong Lee Daniel H. J. Ng Ai, Ling Ho Mohd Rosni Sulaiman Fook, Yee Chye Antioxidants Discovery for Differentiation of Monofloral Stingless Bee Honeys Using Ambient Mass Spectrometry and Metabolomics Approaches education |
| title | Antioxidants Discovery for Differentiation of Monofloral Stingless Bee Honeys Using Ambient Mass Spectrometry and Metabolomics Approaches education |
| title_full | Antioxidants Discovery for Differentiation of Monofloral Stingless Bee Honeys Using Ambient Mass Spectrometry and Metabolomics Approaches education |
| title_fullStr | Antioxidants Discovery for Differentiation of Monofloral Stingless Bee Honeys Using Ambient Mass Spectrometry and Metabolomics Approaches education |
| title_full_unstemmed | Antioxidants Discovery for Differentiation of Monofloral Stingless Bee Honeys Using Ambient Mass Spectrometry and Metabolomics Approaches education |
| title_short | Antioxidants Discovery for Differentiation of Monofloral Stingless Bee Honeys Using Ambient Mass Spectrometry and Metabolomics Approaches education |
| title_sort | antioxidants discovery for differentiation of monofloral stingless bee honeys using ambient mass spectrometry and metabolomics approaches education |
| topic | QP501-801 Animal biochemistry SF521-539.8 Bee culture |
| url | https://eprints.ums.edu.my/id/eprint/38310/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/38310/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/38310/ https://doi.org/10.3390/foods12122404 |
| url_provider | http://eprints.ums.edu.my/ |