A transcriptomic (RNA-seq) analysis of Drosophila melanogaster adult testes overexpressing microRNA-2b-1
MicroRNAs (miRNAs) are short non-coding single-stranded RNAs with approximately 22 nucleotides in length that neg- atively regulate the mRNA translation of a target gene. MiR-2b-1 belongs to the largest miR-2 family in Drosophila melanogaster with 8 members and this miRNA family is con- served in in...
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Elsevier Inc.
2022
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| Online Access: | https://eprints.ums.edu.my/id/eprint/36769/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/36769/2/FULLTEXT.pdf https://eprints.ums.edu.my/id/eprint/36769/ https://doi.org/10.1016/j.dib.2022.108748 |
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| author | Sharvin Manickam Shallinie Thangadurai Azali Azlan Zarina Amin Ghows Azzam Mardani Abdul Halim |
| author_facet | Sharvin Manickam Shallinie Thangadurai Azali Azlan Zarina Amin Ghows Azzam Mardani Abdul Halim |
| author_sort | Sharvin Manickam |
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| continent | Asia |
| country | Malaysia |
| description | MicroRNAs (miRNAs) are short non-coding single-stranded RNAs with approximately 22 nucleotides in length that neg- atively regulate the mRNA translation of a target gene. MiR-2b-1 belongs to the largest miR-2 family in Drosophila melanogaster with 8 members and this miRNA family is con- served in invertebrates. miRNAs play key roles in gene reg- ulation, cell proliferation, cell death, cell differentiation and cell developmental homeostasis in multicellular organisms. Its role in various human diseases is continuously being stud- ied. miRNAs also found out to be crucial in maintaining stem cell niche in D. melanogaster gonads. We have identified that ectopic overexpression of miR-2b-1 of D. melanogaster causes testicular bulging (a tumour like phenotype) in 3-5 days old adult flies. Hence, we have performed a transcriptomic (RNA- seq) analysis to understand the role of miR-2b-1 in the devel- opment, maintenance, and differentiation of D. melanogaster adult testis stem cells. Data are available from GEO (accession number GSE211399). |
| format | Article |
| id | my.ums.eprints-36769 |
| institution | Universiti Malaysia Sabah |
| language | en en |
| publishDate | 2022 |
| publisher | Elsevier Inc. |
| record_format | eprints |
| spelling | my.ums.eprints-367692023-09-14T01:25:23Z https://eprints.ums.edu.my/id/eprint/36769/ A transcriptomic (RNA-seq) analysis of Drosophila melanogaster adult testes overexpressing microRNA-2b-1 Sharvin Manickam Shallinie Thangadurai Azali Azlan Zarina Amin Ghows Azzam Mardani Abdul Halim QP501-801 Animal biochemistry MicroRNAs (miRNAs) are short non-coding single-stranded RNAs with approximately 22 nucleotides in length that neg- atively regulate the mRNA translation of a target gene. MiR-2b-1 belongs to the largest miR-2 family in Drosophila melanogaster with 8 members and this miRNA family is con- served in invertebrates. miRNAs play key roles in gene reg- ulation, cell proliferation, cell death, cell differentiation and cell developmental homeostasis in multicellular organisms. Its role in various human diseases is continuously being stud- ied. miRNAs also found out to be crucial in maintaining stem cell niche in D. melanogaster gonads. We have identified that ectopic overexpression of miR-2b-1 of D. melanogaster causes testicular bulging (a tumour like phenotype) in 3-5 days old adult flies. Hence, we have performed a transcriptomic (RNA- seq) analysis to understand the role of miR-2b-1 in the devel- opment, maintenance, and differentiation of D. melanogaster adult testis stem cells. Data are available from GEO (accession number GSE211399). Elsevier Inc. 2022-11-13 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/36769/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/36769/2/FULLTEXT.pdf Sharvin Manickam and Shallinie Thangadurai and Azali Azlan and Zarina Amin and Ghows Azzam and Mardani Abdul Halim (2022) A transcriptomic (RNA-seq) analysis of Drosophila melanogaster adult testes overexpressing microRNA-2b-1. Data in Brief, 45. pp. 1-6. https://doi.org/10.1016/j.dib.2022.108748 |
| spellingShingle | QP501-801 Animal biochemistry Sharvin Manickam Shallinie Thangadurai Azali Azlan Zarina Amin Ghows Azzam Mardani Abdul Halim A transcriptomic (RNA-seq) analysis of Drosophila melanogaster adult testes overexpressing microRNA-2b-1 |
| title | A transcriptomic (RNA-seq) analysis of Drosophila melanogaster adult testes overexpressing microRNA-2b-1 |
| title_full | A transcriptomic (RNA-seq) analysis of Drosophila melanogaster adult testes overexpressing microRNA-2b-1 |
| title_fullStr | A transcriptomic (RNA-seq) analysis of Drosophila melanogaster adult testes overexpressing microRNA-2b-1 |
| title_full_unstemmed | A transcriptomic (RNA-seq) analysis of Drosophila melanogaster adult testes overexpressing microRNA-2b-1 |
| title_short | A transcriptomic (RNA-seq) analysis of Drosophila melanogaster adult testes overexpressing microRNA-2b-1 |
| title_sort | transcriptomic (rna-seq) analysis of drosophila melanogaster adult testes overexpressing microrna-2b-1 |
| topic | QP501-801 Animal biochemistry |
| url | https://eprints.ums.edu.my/id/eprint/36769/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/36769/2/FULLTEXT.pdf https://eprints.ums.edu.my/id/eprint/36769/ https://doi.org/10.1016/j.dib.2022.108748 |
| url_provider | http://eprints.ums.edu.my/ |