Root distribution in intercropping systems – a comparison of DNA based methods and visual distinction of roots
This study was performed to i) compare root distribution of legume/nonlegume intercrops determined by qPCR and amplicon sequencing (Metabarcoding) to root growth of the individual species determined visually, and ii) compare the qPCR and amplicon sequencing techniques as a method for quantifying roo...
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Informa UK Limited
2019
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| Online Access: | https://eprints.ums.edu.my/id/eprint/34021/1/Root%20distribution%20in%20intercropping%20systems%20%E2%80%93%20a%20comparison%20of%20DNA%20based%20methods%20and%20visual%20distinction%20of%20roots.pdf https://eprints.ums.edu.my/id/eprint/34021/2/Root%20distribution%20in%20intercropping%20systems%20%E2%80%93%20a%20comparison%20of%20DNA%20based%20methods%20and%20visual%20distinction%20of%20roots1.pdf https://eprints.ums.edu.my/id/eprint/34021/ https://www.tandfonline.com/doi/full/10.1080/03650340.2019.1675872 https://doi.org/10.1080/03650340.2019.1675872 |
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| author | Affendy Hassan Dorte Bodin Dresbølla Camilla Ruø Rasmussen Annemette Lyhne-Kjærbye Mette Haubjerg Nicolaisen Michaela Schiller Stokholm Ole Søgaard Lund Kristian Thorup-Kristensen |
| author_facet | Affendy Hassan Dorte Bodin Dresbølla Camilla Ruø Rasmussen Annemette Lyhne-Kjærbye Mette Haubjerg Nicolaisen Michaela Schiller Stokholm Ole Søgaard Lund Kristian Thorup-Kristensen |
| author_sort | Affendy Hassan |
| building | UMS Library |
| collection | Institutional Repository |
| content_provider | Universiti Malaysia Sabah |
| content_source | UMS Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | This study was performed to i) compare root distribution of legume/nonlegume intercrops determined by qPCR and amplicon sequencing (Metabarcoding) to root growth of the individual species determined visually, and ii) compare the qPCR and amplicon sequencing techniques as a method for quantifying root growth of individual species grown in mixtures. Red beet (Beta vulgaris L. cv Detroit) and lucerne (Medicago sativa L. cv Creno) were grown in one-meter-high transparent tubes. Root competition was studied by direct observation on the tube surface determining root intensity (RI), root wash and extraction followed by determination of root length density (RLD), and DNA-based methods. Intercropping decreased lucerne RI strongly, whereas the RI of red beet was not affected. The determination of RLD did not allow us to distinguish species in mixed samples. However, both DNA methods showed apparently a tendency to overestimate the fraction of lucerne roots compared to the direct root observations. The discrepancy between methods was explained by the fact that visual methods provide estimates of root length while the DNA methods provide estimates of root mass. In conclusion, DNA-based estimates of species fractions in mixed root samples give valuable information on root interactions in mixed crops. |
| format | Article |
| id | my.ums.eprints-34021 |
| institution | Universiti Malaysia Sabah |
| language | en en |
| publishDate | 2019 |
| publisher | Informa UK Limited |
| record_format | eprints |
| spelling | my.ums.eprints-340212022-08-29T02:59:44Z https://eprints.ums.edu.my/id/eprint/34021/ Root distribution in intercropping systems – a comparison of DNA based methods and visual distinction of roots Affendy Hassan Dorte Bodin Dresbølla Camilla Ruø Rasmussen Annemette Lyhne-Kjærbye Mette Haubjerg Nicolaisen Michaela Schiller Stokholm Ole Søgaard Lund Kristian Thorup-Kristensen QK640-(707) Plant anatomy This study was performed to i) compare root distribution of legume/nonlegume intercrops determined by qPCR and amplicon sequencing (Metabarcoding) to root growth of the individual species determined visually, and ii) compare the qPCR and amplicon sequencing techniques as a method for quantifying root growth of individual species grown in mixtures. Red beet (Beta vulgaris L. cv Detroit) and lucerne (Medicago sativa L. cv Creno) were grown in one-meter-high transparent tubes. Root competition was studied by direct observation on the tube surface determining root intensity (RI), root wash and extraction followed by determination of root length density (RLD), and DNA-based methods. Intercropping decreased lucerne RI strongly, whereas the RI of red beet was not affected. The determination of RLD did not allow us to distinguish species in mixed samples. However, both DNA methods showed apparently a tendency to overestimate the fraction of lucerne roots compared to the direct root observations. The discrepancy between methods was explained by the fact that visual methods provide estimates of root length while the DNA methods provide estimates of root mass. In conclusion, DNA-based estimates of species fractions in mixed root samples give valuable information on root interactions in mixed crops. Informa UK Limited 2019 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34021/1/Root%20distribution%20in%20intercropping%20systems%20%E2%80%93%20a%20comparison%20of%20DNA%20based%20methods%20and%20visual%20distinction%20of%20roots.pdf text en https://eprints.ums.edu.my/id/eprint/34021/2/Root%20distribution%20in%20intercropping%20systems%20%E2%80%93%20a%20comparison%20of%20DNA%20based%20methods%20and%20visual%20distinction%20of%20roots1.pdf Affendy Hassan and Dorte Bodin Dresbølla and Camilla Ruø Rasmussen and Annemette Lyhne-Kjærbye and Mette Haubjerg Nicolaisen and Michaela Schiller Stokholm and Ole Søgaard Lund and Kristian Thorup-Kristensen (2019) Root distribution in intercropping systems – a comparison of DNA based methods and visual distinction of roots. Archives of Agronomy And Soil Science, 67 (1). pp. 15-28. ISSN 0365-0340 https://www.tandfonline.com/doi/full/10.1080/03650340.2019.1675872 https://doi.org/10.1080/03650340.2019.1675872 https://doi.org/10.1080/03650340.2019.1675872 |
| spellingShingle | QK640-(707) Plant anatomy Affendy Hassan Dorte Bodin Dresbølla Camilla Ruø Rasmussen Annemette Lyhne-Kjærbye Mette Haubjerg Nicolaisen Michaela Schiller Stokholm Ole Søgaard Lund Kristian Thorup-Kristensen Root distribution in intercropping systems – a comparison of DNA based methods and visual distinction of roots |
| title | Root distribution in intercropping systems – a comparison of DNA based methods and visual distinction of roots |
| title_full | Root distribution in intercropping systems – a comparison of DNA based methods and visual distinction of roots |
| title_fullStr | Root distribution in intercropping systems – a comparison of DNA based methods and visual distinction of roots |
| title_full_unstemmed | Root distribution in intercropping systems – a comparison of DNA based methods and visual distinction of roots |
| title_short | Root distribution in intercropping systems – a comparison of DNA based methods and visual distinction of roots |
| title_sort | root distribution in intercropping systems – a comparison of dna based methods and visual distinction of roots |
| topic | QK640-(707) Plant anatomy |
| url | https://eprints.ums.edu.my/id/eprint/34021/1/Root%20distribution%20in%20intercropping%20systems%20%E2%80%93%20a%20comparison%20of%20DNA%20based%20methods%20and%20visual%20distinction%20of%20roots.pdf https://eprints.ums.edu.my/id/eprint/34021/2/Root%20distribution%20in%20intercropping%20systems%20%E2%80%93%20a%20comparison%20of%20DNA%20based%20methods%20and%20visual%20distinction%20of%20roots1.pdf https://eprints.ums.edu.my/id/eprint/34021/ https://www.tandfonline.com/doi/full/10.1080/03650340.2019.1675872 https://doi.org/10.1080/03650340.2019.1675872 https://doi.org/10.1080/03650340.2019.1675872 |
| url_provider | http://eprints.ums.edu.my/ |