Microbial growth rate and distribution of doubling time at different concentration of oil sludge medium
Microorganisms play a critical part in the development of a sustainable ecosystem and biosphere. The generation time, which differs among bacteria is influenced by many environmental factors as well as the nature of the bacteria species. In this present work, the growth curves of five consortia loca...
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2022
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| Online Access: | https://eprints.ums.edu.my/id/eprint/33656/2/Microbial%20growth%20rate%20and%20distribution%20of%20doubling%20time%20at%20different%20concentration%20of%20oil%20sludge%20medium.ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/33656/3/Microbial%20growth%20rate%20and%20distribution%20of%20doubling%20time%20at%20different%20concentration%20of%20oil%20sludge%20medium.pdf https://eprints.ums.edu.my/id/eprint/33656/ https://iopscience.iop.org/article/10.1088/1755-1315/1022/1/012075 |
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| author | Nur Zaida Zahari P M Tuah Siti Aishah Mohd Ali Mohd Khalizan Sabullah |
| author_facet | Nur Zaida Zahari P M Tuah Siti Aishah Mohd Ali Mohd Khalizan Sabullah |
| author_sort | Nur Zaida Zahari |
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| continent | Asia |
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| description | Microorganisms play a critical part in the development of a sustainable ecosystem and biosphere. The generation time, which differs among bacteria is influenced by many environmental factors as well as the nature of the bacteria species. In this present work, the growth curves of five consortia locally isolated beneficial microorganisms (LIBeM) in mixtures of (LIBeM) Candida tropicalis-RETL-Cr1, Chromobacterium violaceum-MAB-Cr1, Pseudomonas aeruginosa-BAS-Cr1, Sphingomonas paucimobilis-RETOS-Cr1, and Stenotrophomonas maltophilia-RAS-Cr1 were reported. A laboratory scale was conducted to observe the doubling time (dt) observed for each consortium LIBeM at varied concentration levels of oil sludge (2%, 5%, 10%, 15% and 20%) v/v. After 24 hours of incubation, the growth of microorganisms was determined using spectrophotometer at 600nm and the standard growth profile were plotted according to the concentrations studied. The results showed that consortia LIBeM at 2% and 5% v/v oil sludge showed the similar growth pattern in the sigmoid curve over a 24-hour period. However, at 10%, 15%, and 20% (v/v) concentrations, the growth tendency is increased and remains consistent during the incubation period. Study on growth rate and doubling time (dt) had showed that Consortia 3 consists of C. tropicalis –RETL-Cr1+ S. maltophilia-RAS-Cr1+ P. aeruginosa-BAS-Cr1 performed the highest growth rate with 0.16 hour-1 and lowest doubling time (dt) of 4.41. This result is critical for determining the efficiency and tolerance of LIBeM for petroleum degradation at various concentration levels of oil sludge in real-world applications. |
| format | Conference or Workshop Item |
| id | my.ums.eprints-33656 |
| institution | Universiti Malaysia Sabah |
| language | en en |
| publishDate | 2022 |
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| spelling | my.ums.eprints-336562022-08-03T23:24:15Z https://eprints.ums.edu.my/id/eprint/33656/ Microbial growth rate and distribution of doubling time at different concentration of oil sludge medium Nur Zaida Zahari P M Tuah Siti Aishah Mohd Ali Mohd Khalizan Sabullah QR75-99.5 Bacteria Microorganisms play a critical part in the development of a sustainable ecosystem and biosphere. The generation time, which differs among bacteria is influenced by many environmental factors as well as the nature of the bacteria species. In this present work, the growth curves of five consortia locally isolated beneficial microorganisms (LIBeM) in mixtures of (LIBeM) Candida tropicalis-RETL-Cr1, Chromobacterium violaceum-MAB-Cr1, Pseudomonas aeruginosa-BAS-Cr1, Sphingomonas paucimobilis-RETOS-Cr1, and Stenotrophomonas maltophilia-RAS-Cr1 were reported. A laboratory scale was conducted to observe the doubling time (dt) observed for each consortium LIBeM at varied concentration levels of oil sludge (2%, 5%, 10%, 15% and 20%) v/v. After 24 hours of incubation, the growth of microorganisms was determined using spectrophotometer at 600nm and the standard growth profile were plotted according to the concentrations studied. The results showed that consortia LIBeM at 2% and 5% v/v oil sludge showed the similar growth pattern in the sigmoid curve over a 24-hour period. However, at 10%, 15%, and 20% (v/v) concentrations, the growth tendency is increased and remains consistent during the incubation period. Study on growth rate and doubling time (dt) had showed that Consortia 3 consists of C. tropicalis –RETL-Cr1+ S. maltophilia-RAS-Cr1+ P. aeruginosa-BAS-Cr1 performed the highest growth rate with 0.16 hour-1 and lowest doubling time (dt) of 4.41. This result is critical for determining the efficiency and tolerance of LIBeM for petroleum degradation at various concentration levels of oil sludge in real-world applications. 2022 Conference or Workshop Item PeerReviewed text en https://eprints.ums.edu.my/id/eprint/33656/2/Microbial%20growth%20rate%20and%20distribution%20of%20doubling%20time%20at%20different%20concentration%20of%20oil%20sludge%20medium.ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/33656/3/Microbial%20growth%20rate%20and%20distribution%20of%20doubling%20time%20at%20different%20concentration%20of%20oil%20sludge%20medium.pdf Nur Zaida Zahari and P M Tuah and Siti Aishah Mohd Ali and Mohd Khalizan Sabullah (2022) Microbial growth rate and distribution of doubling time at different concentration of oil sludge medium. In: The 6th International Conference on Civil and Environmental Engineering for Sustainability, 15-16 November 2021, Virtual. https://iopscience.iop.org/article/10.1088/1755-1315/1022/1/012075 |
| spellingShingle | QR75-99.5 Bacteria Nur Zaida Zahari P M Tuah Siti Aishah Mohd Ali Mohd Khalizan Sabullah Microbial growth rate and distribution of doubling time at different concentration of oil sludge medium |
| title | Microbial growth rate and distribution of doubling time at different concentration of oil sludge medium |
| title_full | Microbial growth rate and distribution of doubling time at different concentration of oil sludge medium |
| title_fullStr | Microbial growth rate and distribution of doubling time at different concentration of oil sludge medium |
| title_full_unstemmed | Microbial growth rate and distribution of doubling time at different concentration of oil sludge medium |
| title_short | Microbial growth rate and distribution of doubling time at different concentration of oil sludge medium |
| title_sort | microbial growth rate and distribution of doubling time at different concentration of oil sludge medium |
| topic | QR75-99.5 Bacteria |
| url | https://eprints.ums.edu.my/id/eprint/33656/2/Microbial%20growth%20rate%20and%20distribution%20of%20doubling%20time%20at%20different%20concentration%20of%20oil%20sludge%20medium.ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/33656/3/Microbial%20growth%20rate%20and%20distribution%20of%20doubling%20time%20at%20different%20concentration%20of%20oil%20sludge%20medium.pdf https://eprints.ums.edu.my/id/eprint/33656/ https://iopscience.iop.org/article/10.1088/1755-1315/1022/1/012075 |
| url_provider | http://eprints.ums.edu.my/ |