Stem hexane extract of strobilanthes crispus induces apoptosis in triple-negative breast cancer cell line
Strobilanthes crispus is known to possess multiple health beneficial effects and reported to be traditionally used as medicine in several countries. This study was to investigate the anti-proliferative effects of S. crispus leaves and stem extracts on MDA-MB-231 by examining their effects on apoptos...
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Routledge
2021
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| Online Access: | https://eprints.ums.edu.my/id/eprint/31417/1/Stem%20hexane%20extract%20of%20strobilanthes%20crispus%20induces%20apoptosis%20in%20triple-negative%20breast%20cancer%20cell%20line_ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/31417/ https://www.tandfonline.com/doi/abs/10.1080/01635581.2021.1880606 https://doi.org/10.1080/01635581.2021.1880606 |
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| author | Norasyidah Gordani Cheong, Bo Eng Teoh, Peik Lin |
| author_facet | Norasyidah Gordani Cheong, Bo Eng Teoh, Peik Lin |
| author_sort | Norasyidah Gordani |
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| content_provider | Universiti Malaysia Sabah |
| content_source | UMS Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Strobilanthes crispus is known to possess multiple health beneficial effects and reported to be traditionally used as medicine in several countries. This study was to investigate the anti-proliferative effects of S. crispus leaves and stem extracts on MDA-MB-231 by examining their effects on apoptosis pathway. The chemical compounds were extracted from leaves and stems using methanol followed by solvent partitioning. Two extracts were found to prevent MDA-MB-231 cell growth at the IC50 of 45 μg/mL and 60 μg/mL, respectively, for leaf water (LW) and stem hexane (SH) extracts. Results showed that SH extract induces apoptosis by suppressing the protein expression of BCL-2 while the expression of pro-apoptotic proteins such as BAX and caspase nine were unchanged. Decrease of cyclin A2 in SH-treated cells suggested this effect was associated with the dysregulation of cell cycle. However, LW extract showed no effects on apoptosis and cell cycle arrest in the treated cells. Taken together, our results showed SH extract of S. crispus exhibiting their anti-proliferative activities by modulating apoptosis and cell cycle, but the underlying mechanisms exerted by LW extract requires further investigation. |
| format | Article |
| id | my.ums.eprints-31417 |
| institution | Universiti Malaysia Sabah |
| language | en |
| publishDate | 2021 |
| publisher | Routledge |
| record_format | eprints |
| spelling | my.ums.eprints-314172021-12-13T07:12:27Z https://eprints.ums.edu.my/id/eprint/31417/ Stem hexane extract of strobilanthes crispus induces apoptosis in triple-negative breast cancer cell line Norasyidah Gordani Cheong, Bo Eng Teoh, Peik Lin RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens Strobilanthes crispus is known to possess multiple health beneficial effects and reported to be traditionally used as medicine in several countries. This study was to investigate the anti-proliferative effects of S. crispus leaves and stem extracts on MDA-MB-231 by examining their effects on apoptosis pathway. The chemical compounds were extracted from leaves and stems using methanol followed by solvent partitioning. Two extracts were found to prevent MDA-MB-231 cell growth at the IC50 of 45 μg/mL and 60 μg/mL, respectively, for leaf water (LW) and stem hexane (SH) extracts. Results showed that SH extract induces apoptosis by suppressing the protein expression of BCL-2 while the expression of pro-apoptotic proteins such as BAX and caspase nine were unchanged. Decrease of cyclin A2 in SH-treated cells suggested this effect was associated with the dysregulation of cell cycle. However, LW extract showed no effects on apoptosis and cell cycle arrest in the treated cells. Taken together, our results showed SH extract of S. crispus exhibiting their anti-proliferative activities by modulating apoptosis and cell cycle, but the underlying mechanisms exerted by LW extract requires further investigation. Routledge 2021 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/31417/1/Stem%20hexane%20extract%20of%20strobilanthes%20crispus%20induces%20apoptosis%20in%20triple-negative%20breast%20cancer%20cell%20line_ABSTRACT.pdf Norasyidah Gordani and Cheong, Bo Eng and Teoh, Peik Lin (2021) Stem hexane extract of strobilanthes crispus induces apoptosis in triple-negative breast cancer cell line. Nutrition and Cancer. ISSN 0163-5581 (P-ISSN) , 1532-7914 (E-ISSN) https://www.tandfonline.com/doi/abs/10.1080/01635581.2021.1880606 https://doi.org/10.1080/01635581.2021.1880606 https://doi.org/10.1080/01635581.2021.1880606 |
| spellingShingle | RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens Norasyidah Gordani Cheong, Bo Eng Teoh, Peik Lin Stem hexane extract of strobilanthes crispus induces apoptosis in triple-negative breast cancer cell line |
| title | Stem hexane extract of strobilanthes crispus induces apoptosis in triple-negative breast cancer cell line |
| title_full | Stem hexane extract of strobilanthes crispus induces apoptosis in triple-negative breast cancer cell line |
| title_fullStr | Stem hexane extract of strobilanthes crispus induces apoptosis in triple-negative breast cancer cell line |
| title_full_unstemmed | Stem hexane extract of strobilanthes crispus induces apoptosis in triple-negative breast cancer cell line |
| title_short | Stem hexane extract of strobilanthes crispus induces apoptosis in triple-negative breast cancer cell line |
| title_sort | stem hexane extract of strobilanthes crispus induces apoptosis in triple-negative breast cancer cell line |
| topic | RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens |
| url | https://eprints.ums.edu.my/id/eprint/31417/1/Stem%20hexane%20extract%20of%20strobilanthes%20crispus%20induces%20apoptosis%20in%20triple-negative%20breast%20cancer%20cell%20line_ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/31417/ https://www.tandfonline.com/doi/abs/10.1080/01635581.2021.1880606 https://doi.org/10.1080/01635581.2021.1880606 https://doi.org/10.1080/01635581.2021.1880606 |
| url_provider | http://eprints.ums.edu.my/ |