Gallocatechin silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways
This study is designed to investigate the combination of gallocatechin (GC) and silver nanoparticles (AgNPs) for its wound healing ability in diabetic rats. Thirty male Sprague Dawley rats were randomly divided into 5 groups: 1. Normal control rats dressed with blank CGP1; 2. Diabetic rats dressed w...
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| _version_ | 1831794566357843968 |
|---|---|
| author | Tan Pei Yin Nagarjuna Reddy Vendidandalaa Giribabu Nelli Visweswara Rao Pasupuleti Shaik Nyamathulla Seri Intan Mokhtar |
| author_facet | Tan Pei Yin Nagarjuna Reddy Vendidandalaa Giribabu Nelli Visweswara Rao Pasupuleti Shaik Nyamathulla Seri Intan Mokhtar |
| author_sort | Tan Pei Yin |
| building | UMS Library |
| collection | Institutional Repository |
| content_provider | Universiti Malaysia Sabah |
| content_source | UMS Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | This study is designed to investigate the combination of gallocatechin (GC) and silver nanoparticles (AgNPs) for its wound healing ability in diabetic rats. Thirty male Sprague Dawley rats were randomly divided into 5 groups: 1. Normal control rats dressed with blank CGP1; 2. Diabetic rats dressed with blank CGP1; 3. Diabetic rats dressed with 13.06μM of GC; 4. Diabetic rats dressed with 26.12 μM of GC; 5. Diabetic rats dressed with 0.1% silver sulfadiazine patches. GC-AgNPs-CGP dressed diabetic rats showed significant FBG reduction, prevented the body weight losses and reduced the oxidative stress by lowering MDA content and elevated antioxidant enzymes such as SOD, CAT and GPx in wound healing skin of diabetic rats when compared to normal CGP. Besides, mRNA expression of Nrf2, Nqo-1, and Ho-1 was upregulated with downregulated expression of Keap-1 mRNA, which is supported by immunohistochemistry. Furthermore, GC-AgNPs-CGP dressing increased growth factors such as VEGF, EGF, TGF-β, and FGF-2 while decreasing MMP-2 in the skin of diabetic wound rats. In vitro permeation study demonstrated rapid GC release and permeation with a flux of 0.061 and 0.143 mg/sq.cm/h. In conclusion, the results indicated that GC-AgNPs-CGP dressing on diabetic wound rats modulated oxidative stress and inflammation with elevated growth factors; increased collagen synthesis thereby significantly improved the wound healing and could be beneficial for the management of diabetic wounds. |
| format | Article |
| id | my.ums.eprints-31270 |
| institution | Universiti Malaysia Sabah |
| language | en en |
| publishDate | 2021 |
| publisher | Elsevier Inc. |
| record_format | eprints |
| spelling | my.ums.eprints-312702021-11-29T08:59:23Z https://eprints.ums.edu.my/id/eprint/31270/ Gallocatechin silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways Tan Pei Yin Nagarjuna Reddy Vendidandalaa Giribabu Nelli Visweswara Rao Pasupuleti Shaik Nyamathulla Seri Intan Mokhtar RA1-1270 Public aspects of medicine RC620-627 Nutritional diseases. Deficiency diseases This study is designed to investigate the combination of gallocatechin (GC) and silver nanoparticles (AgNPs) for its wound healing ability in diabetic rats. Thirty male Sprague Dawley rats were randomly divided into 5 groups: 1. Normal control rats dressed with blank CGP1; 2. Diabetic rats dressed with blank CGP1; 3. Diabetic rats dressed with 13.06μM of GC; 4. Diabetic rats dressed with 26.12 μM of GC; 5. Diabetic rats dressed with 0.1% silver sulfadiazine patches. GC-AgNPs-CGP dressed diabetic rats showed significant FBG reduction, prevented the body weight losses and reduced the oxidative stress by lowering MDA content and elevated antioxidant enzymes such as SOD, CAT and GPx in wound healing skin of diabetic rats when compared to normal CGP. Besides, mRNA expression of Nrf2, Nqo-1, and Ho-1 was upregulated with downregulated expression of Keap-1 mRNA, which is supported by immunohistochemistry. Furthermore, GC-AgNPs-CGP dressing increased growth factors such as VEGF, EGF, TGF-β, and FGF-2 while decreasing MMP-2 in the skin of diabetic wound rats. In vitro permeation study demonstrated rapid GC release and permeation with a flux of 0.061 and 0.143 mg/sq.cm/h. In conclusion, the results indicated that GC-AgNPs-CGP dressing on diabetic wound rats modulated oxidative stress and inflammation with elevated growth factors; increased collagen synthesis thereby significantly improved the wound healing and could be beneficial for the management of diabetic wounds. Elsevier Inc. 2021 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/31270/1/Gallocatechin%20silver%20nanoparticle%20impregnated%20cotton%20gauze%20patches%20enhance%20wound%20healing%20in%20diabetic%20rats%20by%20suppressing%20oxidative%20stress%20and%20inflammation%20via%20modulating%20the.pdf text en https://eprints.ums.edu.my/id/eprint/31270/3/Gallocatechin%20silver%20nanoparticle%20impregnated%20cotton%20gauze%20patches%20enhance%20wound%20healing%20in%20diabetic%20rats%20by%20suppressing%20oxidative%20stress%20and%20inflammation.pdf Tan Pei Yin and Nagarjuna Reddy Vendidandalaa and Giribabu Nelli and Visweswara Rao Pasupuleti and Shaik Nyamathulla and Seri Intan Mokhtar (2021) Gallocatechin silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways. Life Sciences, 286. p. 1. ISSN 0024-3205 https://www.sciencedirect.com/science/article/abs/pii/S0024320521010067?via%3Dihub https://doi.org/10.1016/j.lfs.2021.120019 https://doi.org/10.1016/j.lfs.2021.120019 |
| spellingShingle | RA1-1270 Public aspects of medicine RC620-627 Nutritional diseases. Deficiency diseases Tan Pei Yin Nagarjuna Reddy Vendidandalaa Giribabu Nelli Visweswara Rao Pasupuleti Shaik Nyamathulla Seri Intan Mokhtar Gallocatechin silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways |
| title | Gallocatechin silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways |
| title_full | Gallocatechin silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways |
| title_fullStr | Gallocatechin silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways |
| title_full_unstemmed | Gallocatechin silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways |
| title_short | Gallocatechin silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways |
| title_sort | gallocatechin silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the nrf2/ho-1 and tlr4/nf-κb pathways |
| topic | RA1-1270 Public aspects of medicine RC620-627 Nutritional diseases. Deficiency diseases |
| url | https://eprints.ums.edu.my/id/eprint/31270/1/Gallocatechin%20silver%20nanoparticle%20impregnated%20cotton%20gauze%20patches%20enhance%20wound%20healing%20in%20diabetic%20rats%20by%20suppressing%20oxidative%20stress%20and%20inflammation%20via%20modulating%20the.pdf https://eprints.ums.edu.my/id/eprint/31270/3/Gallocatechin%20silver%20nanoparticle%20impregnated%20cotton%20gauze%20patches%20enhance%20wound%20healing%20in%20diabetic%20rats%20by%20suppressing%20oxidative%20stress%20and%20inflammation.pdf https://eprints.ums.edu.my/id/eprint/31270/ https://www.sciencedirect.com/science/article/abs/pii/S0024320521010067?via%3Dihub https://doi.org/10.1016/j.lfs.2021.120019 https://doi.org/10.1016/j.lfs.2021.120019 |
| url_provider | http://eprints.ums.edu.my/ |