Preparation and characterization of squid pen chitooligosaccharide-epigallocatechin gallate conjugates and their antioxidant and antimicrobial activities
Chitooligosaccharide (COS) and epigallocatechin-3-gallate (EGCG) at various concentrations were used for the preparation of COS–EGCG conjugates. The highest total phenolic content (TPC), representing the amount of EGCG conjugated, was obtained for 1 wt% COS together with EGCG at 0.5 wt% (C1-E0.5- co...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | en en |
| Published: |
The Royal Society of Chemistry 2020
2020
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| Online Access: | https://eprints.ums.edu.my/id/eprint/26229/1/Preparation%20and%20characterization%20of%20squid%20pen%20chitooligosaccharide%E2%80%93epigallocatechin%20gallate%20conjugates%20and%20their%20antioxidant%20and%20antimicrobial%20activities.pdf https://eprints.ums.edu.my/id/eprint/26229/2/Preparation%20and%20characterization%20of%20squid%20pen%20chitooligosaccharide%E2%80%93epigallocatechin%20gallate%20conjugates%20and%20their%20antioxidant%20and%20antimicrobial%20activities1.pdf https://eprints.ums.edu.my/id/eprint/26229/ https://doi.org/10.1039/d0ra05548d |
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| Summary: | Chitooligosaccharide (COS) and epigallocatechin-3-gallate (EGCG) at various concentrations were used for the preparation of COS–EGCG conjugates. The highest total phenolic content (TPC), representing the amount of EGCG conjugated, was obtained for 1 wt% COS together with EGCG at 0.5 wt% (C1-E0.5- conjugate) or 1.0 wt% (C1-E1.0-conjugate) (66.83 and 69.22 mg EGCG per g sample, respectively) (p < 0.05). The 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and 2,20 -azino-bis(3-ethylbenzothiazoline-6- sulfonic acid) (ABTS) radical scavenging activities (DRSA and ARSA, respectively) and ferric reducing antioxidant power (FRAP) of all the samples showed similar trends with TPC. The C1-E0.5-conjugate had higher DRSA, ARSA, FRAP and oxygen radical absorbance capacity (ORAC) values than COS (p < 0.05). Similarly, the antimicrobial activity of COS increased when conjugated with EGCG (p < 0.05). FTIR, 1 HNMR and 13C-NMR analyses confirmed the successful grafting of EGCG with COS. Therefore, 1 wt% COS and 0.5 wt% EGCG were used for the production of a conjugate with augmented antioxidant activity, which could be used to retard lipid oxidation of fatty foods. |
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