An optimal blend of single auto displayed cellulases for cellulose saccharification – a proof of concept
BACKGOUND: To date, it has been challenging to determine the optimal proportions of the individual cellulases in crude extracts and multienzyme systems to promote enzyme synergism. The application of single surface-display of enzymes may obviate such challenges. This article presents a proof of conc...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | en |
| Published: |
2018
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| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/24196/1/An%20optimal%20blend%20of%20single%20auto%20displayed%20cellulases%20for%20cellulose%20saccharification.pdf https://eprints.ums.edu.my/id/eprint/24196/ http://DOI 10.1002/jctb.5628 |
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| Summary: | BACKGOUND: To date, it has been challenging to determine the optimal proportions of the individual cellulases in crude extracts and multienzyme systems to promote enzyme synergism. The application of single surface-display of enzymes may obviate such challenges. This article presents a proof of concept of the optimal mixing of single surface-displayed cellulases for cellulose hydrolysis using Simplex lattice mixture design. RESULTS: The recently discovered maximized autotransporter-mediated expression system was used to express the three cellulases. The biochemical screening analysis revealed that the enzymes have a broad range of functional pH (4–9) and temperature (30–100 ∘C) characteristics. The evaluated optima were pH 6 and 60 ∘C. Through the statistical design of experiment, a blend ratio of 1: 1.6: 1.4 of endoglucanase: exoglucanase: |
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