Effect of temperature on Candida antartica lipase B activity in the kinetic resolution of acebutolol
Thermodynamic studies of free Candida antartica lipase B in kinetic resolution of acebutolol have been carried out to characterize the temperature effects towards enzyme stability and activity. A decreased in reaction rate was observed in temperature above 40oC. Thermodynamic studies on lipase deact...
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| Main Authors: | , |
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| Format: | Article |
| Language: | en en |
| Published: |
2016
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| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/21629/1/Effect%20of%20temperature%20on%20Candida%20antartica%20lipase%20B%20activity%20in%20the%20kinetic%20resolution%20of%20acebutolol.pdf https://eprints.ums.edu.my/id/eprint/21629/7/Effect%20of%20temperature%20on%20Candida%20antartica%20lipase.pdf https://eprints.ums.edu.my/id/eprint/21629/ |
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| Summary: | Thermodynamic studies of free Candida antartica lipase B in kinetic resolution of acebutolol have been carried out to characterize the temperature effects towards enzyme stability and activity. A decreased in reaction rate was observed in temperature above 40oC. Thermodynamic studies on lipase deactivation exhibited a first-order kinetic pattern. The activation and deactivation energies were 39.63 kJ/mol and 54.90 kJ/mol, respectively. The enthalpy and entropy of the lipase deactivation were found to be 52.12 kJ/mol and -0.18 kJ/mol, respectively. |
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