Epidemiology of two human protoparvoviruses, bufavirus and tusavirus
Two human parvoviruses were recently discovered by metagenomics in Africa, bufavirus (BuV) in 2012 and tusavirus (TuV) in 2014. These viruses have been studied exclusively by PCR in stool and detected only in patients with diarrhoea, although at low prevalence. Three genotypes of BuV have been ident...
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Springer Nature
2016
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| Online Access: | https://eprints.ums.edu.my/id/eprint/19599/1/Epidemiology%20of%20two%20human%20protoparvoviruses.pdf https://eprints.ums.edu.my/id/eprint/19599/7/Epidemiology%20of%20two%20human.pdf https://eprints.ums.edu.my/id/eprint/19599/ http://doi.org/10.1038/srep39267 |
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| author | Elina Vaisanen Minna Paloniemi Inka Kuisma Väinö Lithovius Arun Kumar Rauli Franssila Kamruddin Ahmed Eric Delwart Timo Vesikari Klaus Hedman Maria Söderlund-Venermo |
| author_facet | Elina Vaisanen Minna Paloniemi Inka Kuisma Väinö Lithovius Arun Kumar Rauli Franssila Kamruddin Ahmed Eric Delwart Timo Vesikari Klaus Hedman Maria Söderlund-Venermo |
| author_sort | Elina Vaisanen |
| building | UMS Library |
| collection | Institutional Repository |
| content_provider | Universiti Malaysia Sabah |
| content_source | UMS Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Two human parvoviruses were recently discovered by metagenomics in Africa, bufavirus (BuV) in 2012 and tusavirus (TuV) in 2014. These viruses have been studied exclusively by PCR in stool and detected only in patients with diarrhoea, although at low prevalence. Three genotypes of BuV have been identified. We detected, by in-house EIA, BuV1-3 IgG antibodies in 7/228 children (3.1%) and 10/180 adults (5.6%), whereas TuV IgG was found in one child (0.4%). All children and 91% of the adults were Finnish, yet interestingly 3/6 adults of Indian origin were BuV-IgG positive. By competition EIA, no cross-reactivity between the BuVs was detected, indicating that the BuV genotypes represent distinct serotypes. Furthermore, we analysed by BuV qPCR stool and nasal swab samples from 955 children with gastroenteritis, respiratory illness, or both, and found BuV DNA in three stools (0.3%) and for the first time in a nasal swab (0.1%). This is the first study documenting the presence of BuV and TuV antibodies in humans. Although the seroprevalences of both viruses were low in Finland, our results indicate that BuV infections might be widespread in Asia. The BuV-specific humoral immune responses appeared to be strong and long-lasting, pointing to systemic infection in humans. |
| format | Article |
| id | my.ums.eprints-19599 |
| institution | Universiti Malaysia Sabah |
| language | en en |
| publishDate | 2016 |
| publisher | Springer Nature |
| record_format | eprints |
| spelling | my.ums.eprints-195992020-12-19T02:23:10Z https://eprints.ums.edu.my/id/eprint/19599/ Epidemiology of two human protoparvoviruses, bufavirus and tusavirus Elina Vaisanen Minna Paloniemi Inka Kuisma Väinö Lithovius Arun Kumar Rauli Franssila Kamruddin Ahmed Eric Delwart Timo Vesikari Klaus Hedman Maria Söderlund-Venermo QR Microbiology Two human parvoviruses were recently discovered by metagenomics in Africa, bufavirus (BuV) in 2012 and tusavirus (TuV) in 2014. These viruses have been studied exclusively by PCR in stool and detected only in patients with diarrhoea, although at low prevalence. Three genotypes of BuV have been identified. We detected, by in-house EIA, BuV1-3 IgG antibodies in 7/228 children (3.1%) and 10/180 adults (5.6%), whereas TuV IgG was found in one child (0.4%). All children and 91% of the adults were Finnish, yet interestingly 3/6 adults of Indian origin were BuV-IgG positive. By competition EIA, no cross-reactivity between the BuVs was detected, indicating that the BuV genotypes represent distinct serotypes. Furthermore, we analysed by BuV qPCR stool and nasal swab samples from 955 children with gastroenteritis, respiratory illness, or both, and found BuV DNA in three stools (0.3%) and for the first time in a nasal swab (0.1%). This is the first study documenting the presence of BuV and TuV antibodies in humans. Although the seroprevalences of both viruses were low in Finland, our results indicate that BuV infections might be widespread in Asia. The BuV-specific humoral immune responses appeared to be strong and long-lasting, pointing to systemic infection in humans. Springer Nature 2016 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/19599/1/Epidemiology%20of%20two%20human%20protoparvoviruses.pdf text en https://eprints.ums.edu.my/id/eprint/19599/7/Epidemiology%20of%20two%20human.pdf Elina Vaisanen and Minna Paloniemi and Inka Kuisma and Väinö Lithovius and Arun Kumar and Rauli Franssila and Kamruddin Ahmed and Eric Delwart and Timo Vesikari and Klaus Hedman and Maria Söderlund-Venermo (2016) Epidemiology of two human protoparvoviruses, bufavirus and tusavirus. Scientific Reports, 6 (39267). pp. 1-8. ISSN 2045-2322 http://doi.org/10.1038/srep39267 |
| spellingShingle | QR Microbiology Elina Vaisanen Minna Paloniemi Inka Kuisma Väinö Lithovius Arun Kumar Rauli Franssila Kamruddin Ahmed Eric Delwart Timo Vesikari Klaus Hedman Maria Söderlund-Venermo Epidemiology of two human protoparvoviruses, bufavirus and tusavirus |
| title | Epidemiology of two human protoparvoviruses, bufavirus and tusavirus |
| title_full | Epidemiology of two human protoparvoviruses, bufavirus and tusavirus |
| title_fullStr | Epidemiology of two human protoparvoviruses, bufavirus and tusavirus |
| title_full_unstemmed | Epidemiology of two human protoparvoviruses, bufavirus and tusavirus |
| title_short | Epidemiology of two human protoparvoviruses, bufavirus and tusavirus |
| title_sort | epidemiology of two human protoparvoviruses, bufavirus and tusavirus |
| topic | QR Microbiology |
| url | https://eprints.ums.edu.my/id/eprint/19599/1/Epidemiology%20of%20two%20human%20protoparvoviruses.pdf https://eprints.ums.edu.my/id/eprint/19599/7/Epidemiology%20of%20two%20human.pdf https://eprints.ums.edu.my/id/eprint/19599/ http://doi.org/10.1038/srep39267 |
| url_provider | http://eprints.ums.edu.my/ |