Optimization of bioethanol production from seaweeds (Eucheuma spp.)
The depletion of fossil fuels and increase in energy demand give rise to the urgent need to find a new alternative energy resource. The production of bioethanol using land-based and food crops have brought much controversy and debate on their sustainability. Respective to this, the use of marine...
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| Format: | Academic Exercise |
| Language: | en |
| Published: |
2015
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| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/18958/1/Optimization%20of%20bioethanol%20.pdf https://eprints.ums.edu.my/id/eprint/18958/ |
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| Summary: | The depletion of fossil fuels and increase in energy demand give rise to the urgent
need to find a new alternative energy resource. The production of bioethanol using
land-based and food crops have brought much controversy and debate on their
sustainability. Respective to this, the use of marine biomass such as seaweeds in
bioethanol production provides a possible solution for this energy issue. In this study,
the potential for bioethanol production of the two species that abundantly found in
Sabah, Malaysia: Eucheuma cottonii (Kappaphycus alvarezii) and Eucheuma
denticulatum was studied by determining the amount of reducing sugars after acid
hydrolysis as higher reducing sugars content will increase the potential of higher
bioethanol yield. E. cottonii was selected due to the higher reducing sugars content
and expected to produce higher bioethanol yield during fermentation. E. cottonii
samples were hydrolysed by autoclave using sulphuric acid as catalyst with different
acid concentrations (0.05-0.5 M), hydrolysis temperatures (110-130 °C) and
hydrolysis time (5-25 minutes). The optimum conditions for hydrolysis were achieved
at 0.1 M, 130 °C and 15 minutes. Before fermentation, the microscopic view and
growth of yeast Saccharornyces cerevisiae were studied in the preparation of yeast
inoculum. The yeast cells were harvested at the earliest exponential phase that is
after nine hours of incubation. Subsequently, if. cottonii hydrolysates were fermented
using different concentration of inoculum (10-30 v/v %) and fermentation time (12-
72 hours). Bioethanol content was quantified by using Gas Chromatography- Mass
Spectrometry (GC-MS). The maximum bioethanol concentration (9.331 g/L) and
bioethanol yield (0.164 g/g) were achieved at 20 v/v % yeast inoculum and 24 hours
of fermentation duration. These results may provide useful information for
development of more efficient methods for bioethanol production from seaweeds |
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