Study on factors affecting extraction of carotene from carrot by using soxhlet extraction method

In this research, carotene in the carrot (Daucus carota L.) was extracted by using Soxhlet extraction method. Carotene as a pigment that naturally contained in most vegetables is important source of nutrients. The main constituent of the carotenes which is beta-carotene was investigated. Two variabl...

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Main Author: Mohamad Zaidi, Yahaya
Format: Undergraduates Project Papers
Language:en
Published: 2013
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Online Access:http://umpir.ump.edu.my/id/eprint/9452/1/CD8355.pdf
http://umpir.ump.edu.my/id/eprint/9452/
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author Mohamad Zaidi, Yahaya
author_facet Mohamad Zaidi, Yahaya
author_sort Mohamad Zaidi, Yahaya
building UMPSA Library
collection Institutional Repository
content_provider Universiti Malaysia Pahang Al-Sultan Abdullah
content_source UMPSA Institutional Repository
continent Asia
country Malaysia
description In this research, carotene in the carrot (Daucus carota L.) was extracted by using Soxhlet extraction method. Carotene as a pigment that naturally contained in most vegetables is important source of nutrients. The main constituent of the carotenes which is beta-carotene was investigated. Two variables were varied in order to investigate the effect of drying time and solid-to-solvent ratio on the extraction and beta-carotene yield. Samples with different water content were prepared according to drying time of 3 h, 6 h, 9 h, 12 h, and 24 h at 60°C. The solvent used in the extraction process is 2-propanol with solid-to-solvent ratio of 1:4, 1:6, 1:8, and 1:10. After the extraction process, the mixture of carotene and solvent was separated using rotary evaporator in order to get extracted carotene. Then, the extracted carotene was analyzed using HPLC to determine the beta-carotene yield. From HPLC analysis, it shows the composition of beta-carotene in the extracted carotene increase as increasing drying time and solid-to-solvent ratio. This is due to less presence of water content in the sample and the excess of solvent used enhance the extraction process. The study found that optimal operating condition for this carotene extraction process was obtained at 12 h of drying time and 1:10 of solid-to-solvent ratio.
format Undergraduates Project Papers
id my.ump.umpir.9452
institution Universiti Malaysia Pahang
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publishDate 2013
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spelling my.ump.umpir.94522021-07-06T07:26:47Z http://umpir.ump.edu.my/id/eprint/9452/ Study on factors affecting extraction of carotene from carrot by using soxhlet extraction method Mohamad Zaidi, Yahaya TP Chemical technology In this research, carotene in the carrot (Daucus carota L.) was extracted by using Soxhlet extraction method. Carotene as a pigment that naturally contained in most vegetables is important source of nutrients. The main constituent of the carotenes which is beta-carotene was investigated. Two variables were varied in order to investigate the effect of drying time and solid-to-solvent ratio on the extraction and beta-carotene yield. Samples with different water content were prepared according to drying time of 3 h, 6 h, 9 h, 12 h, and 24 h at 60°C. The solvent used in the extraction process is 2-propanol with solid-to-solvent ratio of 1:4, 1:6, 1:8, and 1:10. After the extraction process, the mixture of carotene and solvent was separated using rotary evaporator in order to get extracted carotene. Then, the extracted carotene was analyzed using HPLC to determine the beta-carotene yield. From HPLC analysis, it shows the composition of beta-carotene in the extracted carotene increase as increasing drying time and solid-to-solvent ratio. This is due to less presence of water content in the sample and the excess of solvent used enhance the extraction process. The study found that optimal operating condition for this carotene extraction process was obtained at 12 h of drying time and 1:10 of solid-to-solvent ratio. 2013-07 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/9452/1/CD8355.pdf Mohamad Zaidi, Yahaya (2013) Study on factors affecting extraction of carotene from carrot by using soxhlet extraction method. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
spellingShingle TP Chemical technology
Mohamad Zaidi, Yahaya
Study on factors affecting extraction of carotene from carrot by using soxhlet extraction method
title Study on factors affecting extraction of carotene from carrot by using soxhlet extraction method
title_full Study on factors affecting extraction of carotene from carrot by using soxhlet extraction method
title_fullStr Study on factors affecting extraction of carotene from carrot by using soxhlet extraction method
title_full_unstemmed Study on factors affecting extraction of carotene from carrot by using soxhlet extraction method
title_short Study on factors affecting extraction of carotene from carrot by using soxhlet extraction method
title_sort study on factors affecting extraction of carotene from carrot by using soxhlet extraction method
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/9452/1/CD8355.pdf
http://umpir.ump.edu.my/id/eprint/9452/
url_provider http://umpir.ump.edu.my/