Impedimetric Graphene-based Biosensor for the Detection of Escherichia Coli DNA
A label-free impedimetric DNA biosensor based on graphene nanosheets has been developed for the detection of Escherichia coli O157:H7 strain GZ-021210. Probe DNA (pDNA) of E. coli was immobilized onto graphene nanosheets by the surface functionalization of graphene with 1-pyrenebutyric acid (PyBA) f...
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| Language: | en |
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Royal Society of Chemistry
2014
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| Online Access: | http://umpir.ump.edu.my/id/eprint/8061/1/Impedimetric_graphene-based_biosensor_for_the_detection_of_Escherichia_coli_DNA_%28published%29.pdf http://umpir.ump.edu.my/id/eprint/8061/ http://dx.doi.org/10.1039/C4AY01836B |
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| author | Nurulasma, Zainudin Mohd Hairul, Ab. Rahim M. M., Yusoff K. F., Chong |
| author_facet | Nurulasma, Zainudin Mohd Hairul, Ab. Rahim M. M., Yusoff K. F., Chong |
| author_sort | Nurulasma, Zainudin |
| building | UMPSA Library |
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| content_provider | Universiti Malaysia Pahang Al-Sultan Abdullah |
| content_source | UMPSA Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | A label-free impedimetric DNA biosensor based on graphene nanosheets has been developed for the detection of Escherichia coli O157:H7 strain GZ-021210. Probe DNA (pDNA) of E. coli was immobilized onto graphene nanosheets by the surface functionalization of graphene with 1-pyrenebutyric acid (PyBA) followed by carbodiimide linkage. The hybridization of complementary DNA (cDNA) of E. coli with the immobilized pDNA increased the electron transfer resistance of the graphene nanosheets, as observed
by electrochemical impedance spectroscopy (EIS). The E. coli DNA biosensor displayed a wide range of linear response (1.0 1010 M to 1.0 1014 M), low detection limit (0.7 1015 M), single-base mismatch selectivity, high robustness and good reproducibility. The current work demonstrates an important advancement in the development of a sensitive biosensor for E. coli detection. |
| format | Article |
| id | my.ump.umpir.8061 |
| institution | Universiti Malaysia Pahang |
| language | en |
| publishDate | 2014 |
| publisher | Royal Society of Chemistry |
| record_format | eprints |
| spelling | my.ump.umpir.80612018-01-22T01:29:43Z http://umpir.ump.edu.my/id/eprint/8061/ Impedimetric Graphene-based Biosensor for the Detection of Escherichia Coli DNA Nurulasma, Zainudin Mohd Hairul, Ab. Rahim M. M., Yusoff K. F., Chong QD Chemistry A label-free impedimetric DNA biosensor based on graphene nanosheets has been developed for the detection of Escherichia coli O157:H7 strain GZ-021210. Probe DNA (pDNA) of E. coli was immobilized onto graphene nanosheets by the surface functionalization of graphene with 1-pyrenebutyric acid (PyBA) followed by carbodiimide linkage. The hybridization of complementary DNA (cDNA) of E. coli with the immobilized pDNA increased the electron transfer resistance of the graphene nanosheets, as observed by electrochemical impedance spectroscopy (EIS). The E. coli DNA biosensor displayed a wide range of linear response (1.0 1010 M to 1.0 1014 M), low detection limit (0.7 1015 M), single-base mismatch selectivity, high robustness and good reproducibility. The current work demonstrates an important advancement in the development of a sensitive biosensor for E. coli detection. Royal Society of Chemistry 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/8061/1/Impedimetric_graphene-based_biosensor_for_the_detection_of_Escherichia_coli_DNA_%28published%29.pdf Nurulasma, Zainudin and Mohd Hairul, Ab. Rahim and M. M., Yusoff and K. F., Chong (2014) Impedimetric Graphene-based Biosensor for the Detection of Escherichia Coli DNA. Analytical Methods, 6 (19). pp. 7935-7941. ISSN 1759-9660 (print); 1759-9679 (online). (Published) http://dx.doi.org/10.1039/C4AY01836B DOI: 10.1039/C4AY01836B |
| spellingShingle | QD Chemistry Nurulasma, Zainudin Mohd Hairul, Ab. Rahim M. M., Yusoff K. F., Chong Impedimetric Graphene-based Biosensor for the Detection of Escherichia Coli DNA |
| title | Impedimetric Graphene-based Biosensor for the
Detection of Escherichia Coli DNA |
| title_full | Impedimetric Graphene-based Biosensor for the
Detection of Escherichia Coli DNA |
| title_fullStr | Impedimetric Graphene-based Biosensor for the
Detection of Escherichia Coli DNA |
| title_full_unstemmed | Impedimetric Graphene-based Biosensor for the
Detection of Escherichia Coli DNA |
| title_short | Impedimetric Graphene-based Biosensor for the
Detection of Escherichia Coli DNA |
| title_sort | impedimetric graphene-based biosensor for the
detection of escherichia coli dna |
| topic | QD Chemistry |
| url | http://umpir.ump.edu.my/id/eprint/8061/1/Impedimetric_graphene-based_biosensor_for_the_detection_of_Escherichia_coli_DNA_%28published%29.pdf http://umpir.ump.edu.my/id/eprint/8061/ http://dx.doi.org/10.1039/C4AY01836B |
| url_provider | http://umpir.ump.edu.my/ |