Regenerated Cellulose Membrane with Different Spacer Arm Length of Diamine Monomer for Membrane Chromatography
Packed bed column chromatography is widely used for protein separation. However, it has several limitations such as high pressure drop and long processing times. Membrane chromatography is an alternative technique used for protein separation. Specific monomer can be grafted to uncharged membrane to...
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2014
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| Online Access: | http://umpir.ump.edu.my/id/eprint/7486/1/Regenerated_Cellulose_Membrane_with_Different_Spacer_Arm_Length_of_Diamine_Monomer_for_Membrane_Chromatography.pdf http://umpir.ump.edu.my/id/eprint/7486/ |
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| author | Yue , Wei Lee Syed Mohd Saufi, Tuan Chik |
| author_facet | Yue , Wei Lee Syed Mohd Saufi, Tuan Chik |
| author_sort | Yue , Wei Lee |
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| country | Malaysia |
| description | Packed bed column chromatography is widely used for protein separation. However, it has several limitations such as high pressure drop and long processing times. Membrane chromatography is an alternative technique used for protein separation. Specific monomer can be grafted to uncharged membrane to transform into membrane chromatography material. Optimization of parameters involved during this chemical modification is crucial for the development of high performance membrane chromatography for protein separation. The purpose of this research is to develop
anionexchanger membrane chromatography from regenerated cellulose membrane by attaching different spacer arm lengths of diamine monomer. Regenerated cellulose membrane was activated in a solution containing sodium hydroxide (NaOH) and epichlorohydrin (EPI). The membrane was grafted with diamine solution of 1,2-diaminoethane or 1,4-diaminobutane to produce positively charged membrane
chromatography. The concentration of NaOH activation from 0.05M to 0.50M and diamine monomer concentration from 0.25M to 2.0M during grafting were studied. The optimum concentration of NaOH was 0.20M which produced anion exchange membrane chromatography with capacity of 0.310±0.033 mgBSA/cm2 membrane. High concentration of diamine monomer at 2.0M 1,4-diaminobutane showed a binding
capacity of 0.385±0.027mgBSA/cm2 membrane |
| format | Article |
| id | my.ump.umpir.7486 |
| institution | Universiti Malaysia Pahang |
| language | en |
| publishDate | 2014 |
| publisher | AENSI |
| record_format | eprints |
| spelling | my.ump.umpir.74862018-04-11T01:59:41Z http://umpir.ump.edu.my/id/eprint/7486/ Regenerated Cellulose Membrane with Different Spacer Arm Length of Diamine Monomer for Membrane Chromatography Yue , Wei Lee Syed Mohd Saufi, Tuan Chik TP Chemical technology Packed bed column chromatography is widely used for protein separation. However, it has several limitations such as high pressure drop and long processing times. Membrane chromatography is an alternative technique used for protein separation. Specific monomer can be grafted to uncharged membrane to transform into membrane chromatography material. Optimization of parameters involved during this chemical modification is crucial for the development of high performance membrane chromatography for protein separation. The purpose of this research is to develop anionexchanger membrane chromatography from regenerated cellulose membrane by attaching different spacer arm lengths of diamine monomer. Regenerated cellulose membrane was activated in a solution containing sodium hydroxide (NaOH) and epichlorohydrin (EPI). The membrane was grafted with diamine solution of 1,2-diaminoethane or 1,4-diaminobutane to produce positively charged membrane chromatography. The concentration of NaOH activation from 0.05M to 0.50M and diamine monomer concentration from 0.25M to 2.0M during grafting were studied. The optimum concentration of NaOH was 0.20M which produced anion exchange membrane chromatography with capacity of 0.310±0.033 mgBSA/cm2 membrane. High concentration of diamine monomer at 2.0M 1,4-diaminobutane showed a binding capacity of 0.385±0.027mgBSA/cm2 membrane AENSI 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7486/1/Regenerated_Cellulose_Membrane_with_Different_Spacer_Arm_Length_of_Diamine_Monomer_for_Membrane_Chromatography.pdf Yue , Wei Lee and Syed Mohd Saufi, Tuan Chik (2014) Regenerated Cellulose Membrane with Different Spacer Arm Length of Diamine Monomer for Membrane Chromatography. Advances in Environmental Biology, 8 (3). pp. 596-602. ISSN 1995-0756(Print); 1998-1066(Online). (Published) |
| spellingShingle | TP Chemical technology Yue , Wei Lee Syed Mohd Saufi, Tuan Chik Regenerated Cellulose Membrane with Different Spacer Arm Length of Diamine Monomer for Membrane Chromatography |
| title | Regenerated Cellulose Membrane with Different Spacer Arm Length of Diamine Monomer for Membrane Chromatography |
| title_full | Regenerated Cellulose Membrane with Different Spacer Arm Length of Diamine Monomer for Membrane Chromatography |
| title_fullStr | Regenerated Cellulose Membrane with Different Spacer Arm Length of Diamine Monomer for Membrane Chromatography |
| title_full_unstemmed | Regenerated Cellulose Membrane with Different Spacer Arm Length of Diamine Monomer for Membrane Chromatography |
| title_short | Regenerated Cellulose Membrane with Different Spacer Arm Length of Diamine Monomer for Membrane Chromatography |
| title_sort | regenerated cellulose membrane with different spacer arm length of diamine monomer for membrane chromatography |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/7486/1/Regenerated_Cellulose_Membrane_with_Different_Spacer_Arm_Length_of_Diamine_Monomer_for_Membrane_Chromatography.pdf http://umpir.ump.edu.my/id/eprint/7486/ |
| url_provider | http://umpir.ump.edu.my/ |