Kinetic Analysis on Cell Growth and Biosynthesis of Poly (3-Hydroxybutyrate) (PHB) in Cupriavidus Necator H16

Poly (3-hydroxybutyrate) (PHB) is a biodegradable polymer that can be synthesized through bacterial fermentation. In this study, Cupriavidus necator H16 was used to synthesize PHB by using jatropha oil as its sole carbon source. Experiments using 12.5 g/L of jatropha oil with the highest PHB accumul...

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Main Authors: Khan, Maksudur R., Reddy Prasad, D. M., Hamidah, Abdullah, Mohidin Batcha, Abeed Fatima
Format: Article
Language:en
en
Published: International Academy Publishing (IAP) 2013
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Online Access:http://umpir.ump.edu.my/id/eprint/6483/1/Kinetic_Analysis_on_Cell_Growth_and_Biosynthesis_of_Poly_%283-Hydroxybutyrate%29_%28PHB%29_in_Cupriavidus_Necator_H16.pdf
http://umpir.ump.edu.my/id/eprint/6483/3/Kinetic_Analysis-maksudur-2013.pdf
http://umpir.ump.edu.my/id/eprint/6483/
http://dx.doi.org/10.7763/IJBBB.2013.V3.268
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author Khan, Maksudur R.
Reddy Prasad, D. M.
Hamidah, Abdullah
Mohidin Batcha, Abeed Fatima
author_facet Khan, Maksudur R.
Reddy Prasad, D. M.
Hamidah, Abdullah
Mohidin Batcha, Abeed Fatima
author_sort Khan, Maksudur R.
building UMPSA Library
collection Institutional Repository
content_provider Universiti Malaysia Pahang Al-Sultan Abdullah
content_source UMPSA Institutional Repository
continent Asia
country Malaysia
description Poly (3-hydroxybutyrate) (PHB) is a biodegradable polymer that can be synthesized through bacterial fermentation. In this study, Cupriavidus necator H16 was used to synthesize PHB by using jatropha oil as its sole carbon source. Experiments using 12.5 g/L of jatropha oil with the highest PHB accumulation of 8.6 g/L at 61.5hr was used to assess its kinetic pattern. Logistic and Leudeking-Piret model was used to evaluate the cell growth and PHB accumulation respectively and the theoretical values obtained corresponded well with the experimental data with slight deviation in cell growth and PHB accumulation at the end of stationary phase.
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institution Universiti Malaysia Pahang
language en
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publishDate 2013
publisher International Academy Publishing (IAP)
record_format eprints
spelling my.ump.umpir.64832018-01-16T01:41:57Z http://umpir.ump.edu.my/id/eprint/6483/ Kinetic Analysis on Cell Growth and Biosynthesis of Poly (3-Hydroxybutyrate) (PHB) in Cupriavidus Necator H16 Khan, Maksudur R. Reddy Prasad, D. M. Hamidah, Abdullah Mohidin Batcha, Abeed Fatima TP Chemical technology Poly (3-hydroxybutyrate) (PHB) is a biodegradable polymer that can be synthesized through bacterial fermentation. In this study, Cupriavidus necator H16 was used to synthesize PHB by using jatropha oil as its sole carbon source. Experiments using 12.5 g/L of jatropha oil with the highest PHB accumulation of 8.6 g/L at 61.5hr was used to assess its kinetic pattern. Logistic and Leudeking-Piret model was used to evaluate the cell growth and PHB accumulation respectively and the theoretical values obtained corresponded well with the experimental data with slight deviation in cell growth and PHB accumulation at the end of stationary phase. International Academy Publishing (IAP) 2013 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/6483/1/Kinetic_Analysis_on_Cell_Growth_and_Biosynthesis_of_Poly_%283-Hydroxybutyrate%29_%28PHB%29_in_Cupriavidus_Necator_H16.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/6483/3/Kinetic_Analysis-maksudur-2013.pdf Khan, Maksudur R. and Reddy Prasad, D. M. and Hamidah, Abdullah and Mohidin Batcha, Abeed Fatima (2013) Kinetic Analysis on Cell Growth and Biosynthesis of Poly (3-Hydroxybutyrate) (PHB) in Cupriavidus Necator H16. International Journal of Bioscience, Biochemistry and Bioinformatics, 3 (5). pp. 516-519. ISSN 2010-3638. (Published) http://dx.doi.org/10.7763/IJBBB.2013.V3.268 DOI: 10.7763/IJBBB.2013.V3.268
spellingShingle TP Chemical technology
Khan, Maksudur R.
Reddy Prasad, D. M.
Hamidah, Abdullah
Mohidin Batcha, Abeed Fatima
Kinetic Analysis on Cell Growth and Biosynthesis of Poly (3-Hydroxybutyrate) (PHB) in Cupriavidus Necator H16
title Kinetic Analysis on Cell Growth and Biosynthesis of Poly (3-Hydroxybutyrate) (PHB) in Cupriavidus Necator H16
title_full Kinetic Analysis on Cell Growth and Biosynthesis of Poly (3-Hydroxybutyrate) (PHB) in Cupriavidus Necator H16
title_fullStr Kinetic Analysis on Cell Growth and Biosynthesis of Poly (3-Hydroxybutyrate) (PHB) in Cupriavidus Necator H16
title_full_unstemmed Kinetic Analysis on Cell Growth and Biosynthesis of Poly (3-Hydroxybutyrate) (PHB) in Cupriavidus Necator H16
title_short Kinetic Analysis on Cell Growth and Biosynthesis of Poly (3-Hydroxybutyrate) (PHB) in Cupriavidus Necator H16
title_sort kinetic analysis on cell growth and biosynthesis of poly (3-hydroxybutyrate) (phb) in cupriavidus necator h16
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/6483/1/Kinetic_Analysis_on_Cell_Growth_and_Biosynthesis_of_Poly_%283-Hydroxybutyrate%29_%28PHB%29_in_Cupriavidus_Necator_H16.pdf
http://umpir.ump.edu.my/id/eprint/6483/3/Kinetic_Analysis-maksudur-2013.pdf
http://umpir.ump.edu.my/id/eprint/6483/
http://dx.doi.org/10.7763/IJBBB.2013.V3.268
url_provider http://umpir.ump.edu.my/