Scaling up of poly (3-hydroxybutyrate) production from oil palm frond juice by Cupriavidus necator (CCUG52238T)
It has been reported that oil palm frond juice (OPF) can be used as the sole renewable, alternative and cheap carbon source for the production of poly(3- hydroxybutyrate), P(3HB) at lab scale study. In order to further investigate the potential of OPF juice for P(3HB) production at larger scale, we...
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2013
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| Online Access: | http://umpir.ump.edu.my/id/eprint/3884/1/fkksa-2013-Mior-scaling_up_of-abstract.pdf http://umpir.ump.edu.my/id/eprint/3884/ |
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| author | Mior Ahmad Khushairi, Mohd Zahari |
| author_facet | Mior Ahmad Khushairi, Mohd Zahari |
| author_sort | Mior Ahmad Khushairi, Mohd Zahari |
| building | UMPSA Library |
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| content_provider | Universiti Malaysia Pahang Al-Sultan Abdullah |
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| continent | Asia |
| country | Malaysia |
| description | It has been reported that oil palm frond juice (OPF) can be used as the sole renewable, alternative and cheap carbon source for the production of poly(3- hydroxybutyrate), P(3HB) at lab scale study. In order to further investigate the potential of OPF juice for P(3HB) production at larger scale, we scaled up the fermentation process from 500 ml shake flask to 20 L bioreactor based on constant volumetric mass transfer coefficient (kLa) of oxygen at both scale. It was interesting to note that the P(3HB) content obtained in this study was almost similar as reported in the previous study both in shake flask and 2 L bioreactor. The P(3HB) content for shake flask fermentation was 45 wt.%, while 44 wt.% and 42 wt.% in 2 L and 20 L bioreactor respectively. Based on the current finding, it can be concluded that the scaling up method based on constant kLa was successful due to the almost similar P(3HB) content obtained in shake flask and bioreactor scale. Overall, it can be postulated that OPF juice can be successfully used as a non-food fermentation feedstock for the production of P(3HB) both in small and large scale fermentation. |
| format | Article |
| id | my.ump.umpir.3884 |
| institution | Universiti Malaysia Pahang |
| language | en |
| publishDate | 2013 |
| record_format | eprints |
| spelling | my.ump.umpir.38842017-08-30T07:28:37Z http://umpir.ump.edu.my/id/eprint/3884/ Scaling up of poly (3-hydroxybutyrate) production from oil palm frond juice by Cupriavidus necator (CCUG52238T) Mior Ahmad Khushairi, Mohd Zahari TP Chemical technology It has been reported that oil palm frond juice (OPF) can be used as the sole renewable, alternative and cheap carbon source for the production of poly(3- hydroxybutyrate), P(3HB) at lab scale study. In order to further investigate the potential of OPF juice for P(3HB) production at larger scale, we scaled up the fermentation process from 500 ml shake flask to 20 L bioreactor based on constant volumetric mass transfer coefficient (kLa) of oxygen at both scale. It was interesting to note that the P(3HB) content obtained in this study was almost similar as reported in the previous study both in shake flask and 2 L bioreactor. The P(3HB) content for shake flask fermentation was 45 wt.%, while 44 wt.% and 42 wt.% in 2 L and 20 L bioreactor respectively. Based on the current finding, it can be concluded that the scaling up method based on constant kLa was successful due to the almost similar P(3HB) content obtained in shake flask and bioreactor scale. Overall, it can be postulated that OPF juice can be successfully used as a non-food fermentation feedstock for the production of P(3HB) both in small and large scale fermentation. 2013 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3884/1/fkksa-2013-Mior-scaling_up_of-abstract.pdf Mior Ahmad Khushairi, Mohd Zahari (2013) Scaling up of poly (3-hydroxybutyrate) production from oil palm frond juice by Cupriavidus necator (CCUG52238T). Research Journal of Chemistry and Environment, 17 (7 ). pp. 18-24. ISSN 0972-0626 (Print), 2278-4527 (online). (Published) |
| spellingShingle | TP Chemical technology Mior Ahmad Khushairi, Mohd Zahari Scaling up of poly (3-hydroxybutyrate) production from oil palm frond juice by Cupriavidus necator (CCUG52238T) |
| title | Scaling up of poly (3-hydroxybutyrate) production from oil palm frond juice by Cupriavidus necator (CCUG52238T) |
| title_full | Scaling up of poly (3-hydroxybutyrate) production from oil palm frond juice by Cupriavidus necator (CCUG52238T) |
| title_fullStr | Scaling up of poly (3-hydroxybutyrate) production from oil palm frond juice by Cupriavidus necator (CCUG52238T) |
| title_full_unstemmed | Scaling up of poly (3-hydroxybutyrate) production from oil palm frond juice by Cupriavidus necator (CCUG52238T) |
| title_short | Scaling up of poly (3-hydroxybutyrate) production from oil palm frond juice by Cupriavidus necator (CCUG52238T) |
| title_sort | scaling up of poly (3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (ccug52238t) |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/3884/1/fkksa-2013-Mior-scaling_up_of-abstract.pdf http://umpir.ump.edu.my/id/eprint/3884/ |
| url_provider | http://umpir.ump.edu.my/ |