Production and Optimization of L-asparaginase by Bacillus sp. KK2S4 From Corn Cob
L-Asparaginase (L-asparagine amido hydrolase, E.C.3.5.1.1) is an important enzyme which has anti-tumor properties. This paper describes the production and optimization of L-asparaginase by Bacillus sp. KK2S4 utilization of ground corn cob waste as substrate to reduce and manage the waste in our envi...
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| Language: | en |
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African Journal of Biotechnology
2013
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| Online Access: | http://umpir.ump.edu.my/id/eprint/3780/1/Makky_fist.pdf http://umpir.ump.edu.my/id/eprint/3780/ http://www.academicjournals.org/ajb/abstracts/abs2013/8May/Makky%20et%20al.htm |
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| author | Makky, Essam A. Jee, Jian Ong Karim, Md. Rezaul Lee, Chin Mei |
| author_facet | Makky, Essam A. Jee, Jian Ong Karim, Md. Rezaul Lee, Chin Mei |
| author_sort | Makky, Essam A. |
| building | UMPSA Library |
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| content_provider | Universiti Malaysia Pahang Al-Sultan Abdullah |
| content_source | UMPSA Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | L-Asparaginase (L-asparagine amido hydrolase, E.C.3.5.1.1) is an important enzyme which has anti-tumor properties. This paper describes the production and optimization of L-asparaginase by Bacillus sp. KK2S4 utilization of ground corn cob waste as substrate to reduce and manage the waste in our environment. Thirty-nine (39) bacterial isolates were screened for their L-asparaginase productivity and protein content. Only Bacillus sp KK2S4 was identified before and selected as the most potent isolate. Modified M9 (MM9) minimal complete medium (glucose free) was used as production media for enzyme production. The highest enzyme production was detected at pH 5.0 in presence of lactose and NaNO3 as the best carbon and nitrogen sources with high activity increasing percentage of 1058.9, 377.3 and 566.7%, respectively. |
| format | Article |
| id | my.ump.umpir.3780 |
| institution | Universiti Malaysia Pahang |
| language | en |
| publishDate | 2013 |
| publisher | African Journal of Biotechnology |
| record_format | eprints |
| spelling | my.ump.umpir.37802017-08-14T04:15:44Z http://umpir.ump.edu.my/id/eprint/3780/ Production and Optimization of L-asparaginase by Bacillus sp. KK2S4 From Corn Cob Makky, Essam A. Jee, Jian Ong Karim, Md. Rezaul Lee, Chin Mei TP Chemical technology L-Asparaginase (L-asparagine amido hydrolase, E.C.3.5.1.1) is an important enzyme which has anti-tumor properties. This paper describes the production and optimization of L-asparaginase by Bacillus sp. KK2S4 utilization of ground corn cob waste as substrate to reduce and manage the waste in our environment. Thirty-nine (39) bacterial isolates were screened for their L-asparaginase productivity and protein content. Only Bacillus sp KK2S4 was identified before and selected as the most potent isolate. Modified M9 (MM9) minimal complete medium (glucose free) was used as production media for enzyme production. The highest enzyme production was detected at pH 5.0 in presence of lactose and NaNO3 as the best carbon and nitrogen sources with high activity increasing percentage of 1058.9, 377.3 and 566.7%, respectively. African Journal of Biotechnology 2013-05-08 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3780/1/Makky_fist.pdf Makky, Essam A. and Jee, Jian Ong and Karim, Md. Rezaul and Lee, Chin Mei (2013) Production and Optimization of L-asparaginase by Bacillus sp. KK2S4 From Corn Cob. African Journal of Biotechnology, 12 (19). pp. 2654-2658. ISSN 1684-5315. (Published) http://www.academicjournals.org/ajb/abstracts/abs2013/8May/Makky%20et%20al.htm |
| spellingShingle | TP Chemical technology Makky, Essam A. Jee, Jian Ong Karim, Md. Rezaul Lee, Chin Mei Production and Optimization of L-asparaginase by Bacillus sp. KK2S4 From Corn Cob |
| title | Production and Optimization of L-asparaginase by Bacillus sp. KK2S4 From Corn Cob |
| title_full | Production and Optimization of L-asparaginase by Bacillus sp. KK2S4 From Corn Cob |
| title_fullStr | Production and Optimization of L-asparaginase by Bacillus sp. KK2S4 From Corn Cob |
| title_full_unstemmed | Production and Optimization of L-asparaginase by Bacillus sp. KK2S4 From Corn Cob |
| title_short | Production and Optimization of L-asparaginase by Bacillus sp. KK2S4 From Corn Cob |
| title_sort | production and optimization of l-asparaginase by bacillus sp. kk2s4 from corn cob |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/3780/1/Makky_fist.pdf http://umpir.ump.edu.my/id/eprint/3780/ http://www.academicjournals.org/ajb/abstracts/abs2013/8May/Makky%20et%20al.htm |
| url_provider | http://umpir.ump.edu.my/ |