Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods
Objective: To evaluate the immunosuppressive effect on human phagocytes and antibacterial activity of dihydromorin and norartocarpetin isolated from Artocarpus heterophyllus heartwoods. Methods: Dihydromorin and norartocarpetin were isolated from Artocarpus heterophyllus heartwoods. A modified Boyde...
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Wolters Kluwer Medknow Publications
2020
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| Online Access: | http://umpir.ump.edu.my/id/eprint/30864/1/Immunosuppressive%20and%20antibacterial%20activities%20of%20dihydromorin%20and%20norartocarpetin.pdf http://umpir.ump.edu.my/id/eprint/30864/ https://doi.org/10.4103/2221-1691.287162 |
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| author | Abdi Wira, Septama Ibrahim, Jantan Panichayupakaranant, Pharkphoom Mohd F. F., Mohd Aluwi Eldiza Puji, Rahmi |
| author_facet | Abdi Wira, Septama Ibrahim, Jantan Panichayupakaranant, Pharkphoom Mohd F. F., Mohd Aluwi Eldiza Puji, Rahmi |
| author_sort | Abdi Wira, Septama |
| building | UMPSA Library |
| collection | Institutional Repository |
| content_provider | Universiti Malaysia Pahang Al-Sultan Abdullah |
| content_source | UMPSA Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Objective: To evaluate the immunosuppressive effect on human phagocytes and antibacterial activity of dihydromorin and norartocarpetin isolated from Artocarpus heterophyllus heartwoods. Methods: Dihydromorin and norartocarpetin were isolated from Artocarpus heterophyllus heartwoods. A modified Boyden chamber was used to determine the chemotactic activity of human phagocyte. The respiratory burst was evaluated by chemiluminescence assay. Myeloperoxidase (MPO) activity was quantified using a colorimetric assay. The broth microdilution method was performed to assess their antibacterial activity. Results: Dihydromorin exhibited potent inhibitory effect on the chemotactic activity of polymorphonuclear neutrophils (PMNs) with an IC50 value of 5.03 μg/mL. Dihydromorin also inhibited reactive oxygen species production of whole blood cells, PMNs, and monocytes with IC50 values of 7.88, 7.59 and 7.24 μg/mL, respectively. Interestingly, dihydromorin also strongly inhibited the MPO activity of PMNs with an IC50 value of 5.24 μg/mL, which was lower than indomethacin (24.6 μg/mL). Molecular docking of dihydromorin and crystal structure of MPO showed that dihydromorin had close interaction with key amino acid residues such as Arg239 and Gln91. Antibacterial activity assay showed that only dihydromorin had a strong effect against Streptococcus pyogenes with MIC and MBC values of 15.62 and 31.25 μg/mL, respectively. Conclusions: The results suggest that dihydromorin could be developed as an anti-inflammatory and antibacterial agent. |
| format | Article |
| id | my.ump.umpir.30864 |
| institution | Universiti Malaysia Pahang |
| language | en |
| publishDate | 2020 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | eprints |
| spelling | my.ump.umpir.308642021-06-30T14:21:15Z http://umpir.ump.edu.my/id/eprint/30864/ Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods Abdi Wira, Septama Ibrahim, Jantan Panichayupakaranant, Pharkphoom Mohd F. F., Mohd Aluwi Eldiza Puji, Rahmi TP Chemical technology Objective: To evaluate the immunosuppressive effect on human phagocytes and antibacterial activity of dihydromorin and norartocarpetin isolated from Artocarpus heterophyllus heartwoods. Methods: Dihydromorin and norartocarpetin were isolated from Artocarpus heterophyllus heartwoods. A modified Boyden chamber was used to determine the chemotactic activity of human phagocyte. The respiratory burst was evaluated by chemiluminescence assay. Myeloperoxidase (MPO) activity was quantified using a colorimetric assay. The broth microdilution method was performed to assess their antibacterial activity. Results: Dihydromorin exhibited potent inhibitory effect on the chemotactic activity of polymorphonuclear neutrophils (PMNs) with an IC50 value of 5.03 μg/mL. Dihydromorin also inhibited reactive oxygen species production of whole blood cells, PMNs, and monocytes with IC50 values of 7.88, 7.59 and 7.24 μg/mL, respectively. Interestingly, dihydromorin also strongly inhibited the MPO activity of PMNs with an IC50 value of 5.24 μg/mL, which was lower than indomethacin (24.6 μg/mL). Molecular docking of dihydromorin and crystal structure of MPO showed that dihydromorin had close interaction with key amino acid residues such as Arg239 and Gln91. Antibacterial activity assay showed that only dihydromorin had a strong effect against Streptococcus pyogenes with MIC and MBC values of 15.62 and 31.25 μg/mL, respectively. Conclusions: The results suggest that dihydromorin could be developed as an anti-inflammatory and antibacterial agent. Wolters Kluwer Medknow Publications 2020-08-01 Article PeerReviewed pdf en cc_by_nc_sa_4 http://umpir.ump.edu.my/id/eprint/30864/1/Immunosuppressive%20and%20antibacterial%20activities%20of%20dihydromorin%20and%20norartocarpetin.pdf Abdi Wira, Septama and Ibrahim, Jantan and Panichayupakaranant, Pharkphoom and Mohd F. F., Mohd Aluwi and Eldiza Puji, Rahmi (2020) Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods. Asian Pacific Journal of Tropical Biomedicine, 10 (8). pp. 361-368. ISSN 2221-1691. (Published) https://doi.org/10.4103/2221-1691.287162 https://doi.org/10.4103/2221-1691.287162 |
| spellingShingle | TP Chemical technology Abdi Wira, Septama Ibrahim, Jantan Panichayupakaranant, Pharkphoom Mohd F. F., Mohd Aluwi Eldiza Puji, Rahmi Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods |
| title | Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods |
| title_full | Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods |
| title_fullStr | Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods |
| title_full_unstemmed | Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods |
| title_short | Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods |
| title_sort | immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/30864/1/Immunosuppressive%20and%20antibacterial%20activities%20of%20dihydromorin%20and%20norartocarpetin.pdf http://umpir.ump.edu.my/id/eprint/30864/ https://doi.org/10.4103/2221-1691.287162 https://doi.org/10.4103/2221-1691.287162 |
| url_provider | http://umpir.ump.edu.my/ |