Screening and optimisation study on antioxidant extracts from selected lamiaceae plants
This thesis deals with screening of antioxidant activity, phenols content and mineral profiles, optimisation of extraction of antioxidants and optimisation of spray drying of boiling extract from the selected Lamiaceae plants. The objective of this thesis was to select the plant materials of the...
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| Format: | Thesis |
| Language: | en |
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2010
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| Online Access: | http://umpir.ump.edu.my/id/eprint/2733/1/MOHD_SHAIFUL_AZMAN_B._ABDUL_RAHIM.PDF http://umpir.ump.edu.my/id/eprint/2733/ |
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| _version_ | 1831521277698899968 |
|---|---|
| author | Mohd Shaiful Azman, Abdul Rahim |
| author_facet | Mohd Shaiful Azman, Abdul Rahim |
| author_sort | Mohd Shaiful Azman, Abdul Rahim |
| building | UMPSA Library |
| collection | Institutional Repository |
| content_provider | Universiti Malaysia Pahang Al-Sultan Abdullah |
| content_source | UMPSA Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | This thesis deals with screening of antioxidant activity, phenols content and
mineral profiles, optimisation of extraction of antioxidants and optimisation of spray
drying of boiling extract from the selected Lamiaceae plants. The objective of this thesis
was to select the plant materials of the selected Lamiaceae species that gave the highest
antioxidant activity, highest phenols content and best mineral profiles. This thesis
presents the optimised levels of experimental variables that gave the highest antioxidant
activity-yield. This thesis also presents the optimum levels of the operational variables
that influence the spray drying of the liquid extract of the Lamiaceae plant. Fresh
Plecranthus amboinicus Lour. Indonesia (PALl) samples were randomly collected from
Jakarta, Indonesia and samples of Plecranthus amboinicus Lour. Malaysia (PALM),
Pogostemon cablin Benth. (PCB), SOlenostemon scutellariodes Benth. Red (SSR) and
Solenostemon scutellariodes Mix Colour (SSMC) were randomly collected from
Kuantan, Malaysia. The extracted samples were assessed using 1,1-diphenyl-2-
picry1hydrazyl (DPPH) free radical scavengiig assay for determination of antioxidant
activity. Folin Ciocalteau method was chosen for total phenols determination, while the
essential minerals were analysed by using atomic absorption spectrometer. The 22
factorial design of experiment was used in the optimisation of extraction of antioxidants
by boiling. The method of Path of Steepest Ascent was used to maximise the
antioxidant activity in the spray dried extract. The obtained results indicated that the
- leaves tissues of PCB samples exhibited the significant 6<0.05) highest antioxidant
activity (63.18%) and highest phenols content (7.64 mg/g fresh weight) while the
highest total minerals content was observed in the PALl leaves (38.41 ppm). For
optimisation of extraction of antioxidant by boiling, the theOretical maximum point has
the boiling temperature (T) at 116.18°C and boiling time duration (t) at 107.81 minutes
which gave an antioxidant activity-yield of 65.153 ' %. The combination of levels of
operational variables where the spray dryer was operated at X1 (temperature) =
177.65°C and X2 (concentration of maltodextrin) = 48.65% gave the highest yield of
antioxidant activity of spray dried extract. This research suggests a chemical basis for
some of the health benefits claimed for in folk medicine and warrant further studies to
develop it as a natural functional food. |
| format | Thesis |
| id | my.ump.umpir.2733 |
| institution | Universiti Malaysia Pahang |
| language | en |
| publishDate | 2010 |
| record_format | eprints |
| spelling | my.ump.umpir.27332015-03-03T07:58:12Z http://umpir.ump.edu.my/id/eprint/2733/ Screening and optimisation study on antioxidant extracts from selected lamiaceae plants Mohd Shaiful Azman, Abdul Rahim TP Chemical technology This thesis deals with screening of antioxidant activity, phenols content and mineral profiles, optimisation of extraction of antioxidants and optimisation of spray drying of boiling extract from the selected Lamiaceae plants. The objective of this thesis was to select the plant materials of the selected Lamiaceae species that gave the highest antioxidant activity, highest phenols content and best mineral profiles. This thesis presents the optimised levels of experimental variables that gave the highest antioxidant activity-yield. This thesis also presents the optimum levels of the operational variables that influence the spray drying of the liquid extract of the Lamiaceae plant. Fresh Plecranthus amboinicus Lour. Indonesia (PALl) samples were randomly collected from Jakarta, Indonesia and samples of Plecranthus amboinicus Lour. Malaysia (PALM), Pogostemon cablin Benth. (PCB), SOlenostemon scutellariodes Benth. Red (SSR) and Solenostemon scutellariodes Mix Colour (SSMC) were randomly collected from Kuantan, Malaysia. The extracted samples were assessed using 1,1-diphenyl-2- picry1hydrazyl (DPPH) free radical scavengiig assay for determination of antioxidant activity. Folin Ciocalteau method was chosen for total phenols determination, while the essential minerals were analysed by using atomic absorption spectrometer. The 22 factorial design of experiment was used in the optimisation of extraction of antioxidants by boiling. The method of Path of Steepest Ascent was used to maximise the antioxidant activity in the spray dried extract. The obtained results indicated that the - leaves tissues of PCB samples exhibited the significant 6<0.05) highest antioxidant activity (63.18%) and highest phenols content (7.64 mg/g fresh weight) while the highest total minerals content was observed in the PALl leaves (38.41 ppm). For optimisation of extraction of antioxidant by boiling, the theOretical maximum point has the boiling temperature (T) at 116.18°C and boiling time duration (t) at 107.81 minutes which gave an antioxidant activity-yield of 65.153 ' %. The combination of levels of operational variables where the spray dryer was operated at X1 (temperature) = 177.65°C and X2 (concentration of maltodextrin) = 48.65% gave the highest yield of antioxidant activity of spray dried extract. This research suggests a chemical basis for some of the health benefits claimed for in folk medicine and warrant further studies to develop it as a natural functional food. 2010-12 Thesis NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/2733/1/MOHD_SHAIFUL_AZMAN_B._ABDUL_RAHIM.PDF Mohd Shaiful Azman, Abdul Rahim (2010) Screening and optimisation study on antioxidant extracts from selected lamiaceae plants. Masters thesis, Universiti Malaysia Pahang (Contributors, UNSPECIFIED: UNSPECIFIED). |
| spellingShingle | TP Chemical technology Mohd Shaiful Azman, Abdul Rahim Screening and optimisation study on antioxidant extracts from selected lamiaceae plants |
| title | Screening and optimisation study on antioxidant extracts from selected lamiaceae plants |
| title_full | Screening and optimisation study on antioxidant extracts from selected lamiaceae plants |
| title_fullStr | Screening and optimisation study on antioxidant extracts from selected lamiaceae plants |
| title_full_unstemmed | Screening and optimisation study on antioxidant extracts from selected lamiaceae plants |
| title_short | Screening and optimisation study on antioxidant extracts from selected lamiaceae plants |
| title_sort | screening and optimisation study on antioxidant extracts from selected lamiaceae plants |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/2733/1/MOHD_SHAIFUL_AZMAN_B._ABDUL_RAHIM.PDF http://umpir.ump.edu.my/id/eprint/2733/ |
| url_provider | http://umpir.ump.edu.my/ |