Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support
This paper demonstrates Pseudomonas cepacia lipase catalyzed hydrolysis of p-nitrophenyl palmitate under irradiation of light with wavelengths of 250–750 nm. The reaction follows Michaelis–Menten Kinetics and the light irradiation increases the overall rate of hydrolysis. Using Lineweaver–Burk plot...
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Springer Berlin Heidelberg
2014
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| Online Access: | http://umpir.ump.edu.my/id/eprint/12375/1/Effect%20of%20visible%20light%20on%20catalytic%20hydrolysis%20.pdf http://umpir.ump.edu.my/id/eprint/12375/2/Effect%20of%20visible%20light%20on%20catalytic%20hydrolysis.pdf http://umpir.ump.edu.my/id/eprint/12375/13/Effect%20Of%20Visible%20Light%20On%20Catalytic%20Hydrolysis%20Of%20P-Nitrophenyl%20Palmitate%20By%20The%20Pseudomonas%20Cepacia%20Lipase%20Immobilized%20On%20Sol%E2%80%93Gel%20Support.pdf http://umpir.ump.edu.my/id/eprint/12375/ http://dx.doi.org/10.1007/s00449-014-1213-6 |
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| author | Ponnarasy, Ganasen Khan, Maksudur R. M., Abul Kalam Mohd Sabri, Mahmud |
| author_facet | Ponnarasy, Ganasen Khan, Maksudur R. M., Abul Kalam Mohd Sabri, Mahmud |
| author_sort | Ponnarasy, Ganasen |
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| content_provider | Universiti Malaysia Pahang Al-Sultan Abdullah |
| content_source | UMPSA Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | This paper demonstrates Pseudomonas cepacia lipase catalyzed hydrolysis of p-nitrophenyl palmitate under irradiation of light with wavelengths of 250–750 nm. The reaction follows Michaelis–Menten Kinetics and the light irradiation increases the overall rate of hydrolysis. Using Lineweaver–Burk plot K M and V max values for the reaction in presence of light are found to be 39.07 and 66.67 mM/min/g, respectively; while for the same reaction under dark condition, the values are 7.08 and 10.21 mM/min/g. The linear form of enzyme dependent rate of reaction confirms that no mass-transfer limitations are present and the reaction is a kinetically controlled enzymatic reaction. |
| format | Article |
| id | my.ump.umpir.12375 |
| institution | Universiti Malaysia Pahang |
| language | en en en |
| publishDate | 2014 |
| publisher | Springer Berlin Heidelberg |
| record_format | eprints |
| spelling | my.ump.umpir.123752017-08-17T03:07:53Z http://umpir.ump.edu.my/id/eprint/12375/ Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support Ponnarasy, Ganasen Khan, Maksudur R. M., Abul Kalam Mohd Sabri, Mahmud TA Engineering (General). Civil engineering (General) TP Chemical technology This paper demonstrates Pseudomonas cepacia lipase catalyzed hydrolysis of p-nitrophenyl palmitate under irradiation of light with wavelengths of 250–750 nm. The reaction follows Michaelis–Menten Kinetics and the light irradiation increases the overall rate of hydrolysis. Using Lineweaver–Burk plot K M and V max values for the reaction in presence of light are found to be 39.07 and 66.67 mM/min/g, respectively; while for the same reaction under dark condition, the values are 7.08 and 10.21 mM/min/g. The linear form of enzyme dependent rate of reaction confirms that no mass-transfer limitations are present and the reaction is a kinetically controlled enzymatic reaction. Springer Berlin Heidelberg 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/12375/1/Effect%20of%20visible%20light%20on%20catalytic%20hydrolysis%20.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/12375/2/Effect%20of%20visible%20light%20on%20catalytic%20hydrolysis.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/12375/13/Effect%20Of%20Visible%20Light%20On%20Catalytic%20Hydrolysis%20Of%20P-Nitrophenyl%20Palmitate%20By%20The%20Pseudomonas%20Cepacia%20Lipase%20Immobilized%20On%20Sol%E2%80%93Gel%20Support.pdf Ponnarasy, Ganasen and Khan, Maksudur R. and M., Abul Kalam and Mohd Sabri, Mahmud (2014) Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support. Bioprocess and Biosystems Engineering, 37 (11). pp. 2353-2359. ISSN 1615-7591 (print); 1615-7605 (online). (Published) http://dx.doi.org/10.1007/s00449-014-1213-6 DOI 10.1007/s00449-014-1213-6 |
| spellingShingle | TA Engineering (General). Civil engineering (General) TP Chemical technology Ponnarasy, Ganasen Khan, Maksudur R. M., Abul Kalam Mohd Sabri, Mahmud Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support |
| title | Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support |
| title_full | Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support |
| title_fullStr | Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support |
| title_full_unstemmed | Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support |
| title_short | Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support |
| title_sort | effect of visible light on catalytic hydrolysis of p-nitrophenyl palmitate by the pseudomonas cepacia lipase immobilized on sol–gel support |
| topic | TA Engineering (General). Civil engineering (General) TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/12375/1/Effect%20of%20visible%20light%20on%20catalytic%20hydrolysis%20.pdf http://umpir.ump.edu.my/id/eprint/12375/2/Effect%20of%20visible%20light%20on%20catalytic%20hydrolysis.pdf http://umpir.ump.edu.my/id/eprint/12375/13/Effect%20Of%20Visible%20Light%20On%20Catalytic%20Hydrolysis%20Of%20P-Nitrophenyl%20Palmitate%20By%20The%20Pseudomonas%20Cepacia%20Lipase%20Immobilized%20On%20Sol%E2%80%93Gel%20Support.pdf http://umpir.ump.edu.my/id/eprint/12375/ http://dx.doi.org/10.1007/s00449-014-1213-6 |
| url_provider | http://umpir.ump.edu.my/ |