Comparative Evaluation Of Total Phenolics And Free Radical Scavenging Activity Of The Aqueous Extracts Of Labisia Pumila Var. Alata From Malaysia And Indonesia

Abstract— Functional food and beverage companies are touting the presence of antioxidants in their products in response to consumer interest in the potential health benefits of antioxidants in the diet. Labisia pumila (LPva) or “Kacip Fatimah” is a popular supplement in the Malaysian functional...

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Main Authors: Iwansyah, Ade Chandra, M. M., Yusoff
Format: Conference or Workshop Item
Language:en
Published: 2011
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/1185/1/rp004_ICBFS2011-S014.pdf
http://umpir.ump.edu.my/id/eprint/1185/
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author Iwansyah, Ade Chandra
M. M., Yusoff
author_facet Iwansyah, Ade Chandra
M. M., Yusoff
author_sort Iwansyah, Ade Chandra
building UMPSA Library
collection Institutional Repository
content_provider Universiti Malaysia Pahang Al-Sultan Abdullah
content_source UMPSA Institutional Repository
continent Asia
country Malaysia
description Abstract— Functional food and beverage companies are touting the presence of antioxidants in their products in response to consumer interest in the potential health benefits of antioxidants in the diet. Labisia pumila (LPva) or “Kacip Fatimah” is a popular supplement in the Malaysian functional food and beverage market. Today, information on its bioactive compounds remains scarce. A comparative evaluation of total phenolics and free radical scavenging activity of the aqueous extract of LPva were studied using Folin – Ciocalteu assay, aluminum chloride assay, ferric ion reducing power assay and DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging assay. The results of total phenolics showed that LP from Tilu Mountain- Bogor (LPvaT) contained the highest average of total phenolics. The ratio of total flavonoids/total phenolics (TFC/TPC) of LP from Halimun-Salak Mountain, Bogor (LPvaB) with is 0.27 and contained the highest average of total flavonoids. Antioxidant activity by FRAP assay shows that LPvaT contained the highest average of ability to reduce the ferric ion-TPTZ complex. DPPH assay showed that EC50 of LPvaT (78.79 μg/mL) was lowest. Based on these values, LPvaT is found to have the highest antioxidant activity.
format Conference or Workshop Item
id my.ump.umpir.1185
institution Universiti Malaysia Pahang
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publishDate 2011
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spelling my.ump.umpir.11852018-01-18T01:45:23Z http://umpir.ump.edu.my/id/eprint/1185/ Comparative Evaluation Of Total Phenolics And Free Radical Scavenging Activity Of The Aqueous Extracts Of Labisia Pumila Var. Alata From Malaysia And Indonesia Iwansyah, Ade Chandra M. M., Yusoff TP Chemical technology QD Chemistry Abstract— Functional food and beverage companies are touting the presence of antioxidants in their products in response to consumer interest in the potential health benefits of antioxidants in the diet. Labisia pumila (LPva) or “Kacip Fatimah” is a popular supplement in the Malaysian functional food and beverage market. Today, information on its bioactive compounds remains scarce. A comparative evaluation of total phenolics and free radical scavenging activity of the aqueous extract of LPva were studied using Folin – Ciocalteu assay, aluminum chloride assay, ferric ion reducing power assay and DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging assay. The results of total phenolics showed that LP from Tilu Mountain- Bogor (LPvaT) contained the highest average of total phenolics. The ratio of total flavonoids/total phenolics (TFC/TPC) of LP from Halimun-Salak Mountain, Bogor (LPvaB) with is 0.27 and contained the highest average of total flavonoids. Antioxidant activity by FRAP assay shows that LPvaT contained the highest average of ability to reduce the ferric ion-TPTZ complex. DPPH assay showed that EC50 of LPvaT (78.79 μg/mL) was lowest. Based on these values, LPvaT is found to have the highest antioxidant activity. 2011 Conference or Workshop Item PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/1185/1/rp004_ICBFS2011-S014.pdf Iwansyah, Ade Chandra and M. M., Yusoff (2011) Comparative Evaluation Of Total Phenolics And Free Radical Scavenging Activity Of The Aqueous Extracts Of Labisia Pumila Var. Alata From Malaysia And Indonesia. In: The 2nd International Conference On Biotechnology And Food Sciences , 01-03 April 2011 , Bali, Indonesia. . (Unpublished) (Unpublished)
spellingShingle TP Chemical technology
QD Chemistry
Iwansyah, Ade Chandra
M. M., Yusoff
Comparative Evaluation Of Total Phenolics And Free Radical Scavenging Activity Of The Aqueous Extracts Of Labisia Pumila Var. Alata From Malaysia And Indonesia
title Comparative Evaluation Of Total Phenolics And Free Radical Scavenging Activity Of The Aqueous Extracts Of Labisia Pumila Var. Alata From Malaysia And Indonesia
title_full Comparative Evaluation Of Total Phenolics And Free Radical Scavenging Activity Of The Aqueous Extracts Of Labisia Pumila Var. Alata From Malaysia And Indonesia
title_fullStr Comparative Evaluation Of Total Phenolics And Free Radical Scavenging Activity Of The Aqueous Extracts Of Labisia Pumila Var. Alata From Malaysia And Indonesia
title_full_unstemmed Comparative Evaluation Of Total Phenolics And Free Radical Scavenging Activity Of The Aqueous Extracts Of Labisia Pumila Var. Alata From Malaysia And Indonesia
title_short Comparative Evaluation Of Total Phenolics And Free Radical Scavenging Activity Of The Aqueous Extracts Of Labisia Pumila Var. Alata From Malaysia And Indonesia
title_sort comparative evaluation of total phenolics and free radical scavenging activity of the aqueous extracts of labisia pumila var. alata from malaysia and indonesia
topic TP Chemical technology
QD Chemistry
url http://umpir.ump.edu.my/id/eprint/1185/1/rp004_ICBFS2011-S014.pdf
http://umpir.ump.edu.my/id/eprint/1185/
url_provider http://umpir.ump.edu.my/