Utilization of Palm Kernel cake for the production of mannanase by an indigenous filamentous fungus, aspergillus niger USM F4 under solid substrate fermentation
A mannanase yielding indigenous fungal culture Aspergillus niger USM F4 which was isolated from decayed palm kernel cake (PKC) collected from a local oil palm processed plant was used in this study. The fungal culture produced high yields of extracellular mannanase under solid-substrate fermentation...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Non-Indexed Article |
| Published: |
2011
|
| Online Access: | http://discol.umk.edu.my/id/eprint/7645/ http://www.ispub.com/journal/the-internet-journal-of-microbiology/volume-9-number-1/utilization-of-palm-kernel-cake-for-the-production-of-mannanase-by-an-indigenous-filamentous-fungus-aspergillus-niger-usm-f4-under-solid-substrate-fermentation.html |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | A mannanase yielding indigenous fungal culture Aspergillus niger USM F4 which was isolated from decayed palm kernel cake (PKC) collected from a local oil palm processed plant was used in this study. The fungal culture produced high yields of extracellular mannanase under solid-substrate fermentation (SSF) using PKC as a substrate. The results obtained from this study revealed that after five days of cultivation period, a maximum extracellular mannanase activity was 433.84 U/ g substrate and 0.96 mg glucosamine/g substrate of fungal growth. The optimized cultural conditions and medium compositions were 10 g PKC with particle size of 0.5 mm, moisture content of 80% (v/w), inoculums size of 1x107 spores/ml, incubation temperature of 30ºC, static (without mixing), 2% (w/w) of molasses and 4% (w/w) of ammonium nitrate. There was 53.68% increment of mannanase production after optimization compared to before optimization of cultural conditions and medium compositions. |
|---|