Synthesis and interaction of b-carotene capped cdse nanoparticles with deoxyrybonucleic acid
The interaction between beta carotene capped CdSe nanoparticles and pBR322 DNA was studied using gel electrophoresis. The results showed that the cleaving ability of β-carotene capped CdSe nanoparticles were reasonably effective on pBR322 DNA. The cleavage of DNA from Form I to Form II was mainly ob...
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| Format: | Undergraduate Final Project Report |
| Published: |
2014
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| Online Access: | http://discol.umk.edu.my/id/eprint/5801/ |
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| Summary: | The interaction between beta carotene capped CdSe nanoparticles and pBR322 DNA was studied using gel electrophoresis. The results showed that the cleaving ability of β-carotene capped CdSe nanoparticles were reasonably effective on pBR322 DNA. The cleavage of DNA from Form I to Form II was mainly observed by the band formation in the gel. The band formed and the intensity of the band proves the ability of (β-carotene capped CdSe nanoparticles as DNA cleaving agent compared to other compounds. Although the β-carotene capped CdSe nanoparticles was able to convert Form I to Form II in a very significant amount, only some portion of the Form Ill was cleaved into linearized DNA (Form Ill). The synthesis of β-carotene capped CdSe nanoparticles also has given the nanoparticles the features such as higher absorbance when examined with uv-visible spectroscopy. The results obtained from the spectroscopic test revealed the shift of absorbance range to higher wavelength. Furthermore the newly synthesized β-carotene capped CdSe nanoparticle was found to be water soluble compound. This feature is important in broader field of synthesis. |
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