Association between TNF-a-308 G/A polymorphism and oral cancer risk among Malaysian Indian and indigenous / Mojtaba Golpich
The primary role of tumor necrosis factor alpha (TNF-α) gene is to regulate immune cells. Dysregulation and, in particular, overproduction of this gene has been found to increase susceptibility to a variety of human diseases such as cancer. The aim of this study is to investigate the association of...
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2011
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| Online Access: | http://studentsrepo.um.edu.my/3790/1/1._Title_page%2C_abstract%2C_content.pdf http://studentsrepo.um.edu.my/3790/2/2._Chapter_1_%E2%80%93_6.pdf http://studentsrepo.um.edu.my/3790/3/3._References.pdf http://studentsrepo.um.edu.my/3790/4/4._Appendices.pdf http://pendeta.um.edu.my/client/default/search/results?qu=Association+between+TNF-a-308+G%2FA+polymorphism+and+oral+cancer+risk&te= http://studentsrepo.um.edu.my/3790/ |
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| author | Golpich, Mojtaba |
| author_facet | Golpich, Mojtaba |
| author_sort | Golpich, Mojtaba |
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| content_provider | Universiti Malaya |
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| continent | Asia |
| country | Malaysia |
| description | The primary role of tumor necrosis factor alpha (TNF-α) gene is to regulate immune cells. Dysregulation and, in particular, overproduction of this gene has been found to increase susceptibility to a variety of human diseases such as cancer. The aim of this study is to investigate the association of single nucleotide polymorphism (SNP) in TNF-α −308 promoter and the risk of oral cancer among the Malaysian Indian and Indigenous population.The study included 143 confirmed oral squamous cell carcinoma (OSCC) (mean age = 63.69 ± 12.84) and 79 healthy controls (mean age = 50.43 ± 16.35). The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was employed to analyze TNF-α −308 promoter polymorphism, which were confirmed by direct sequencing.
Chi-square, simple logistic regression and stratified analysis were performed using the SPSS (ver 15.0) to study the role of TNF-α polymorphism in modulating the risk of oral cancer. The wild-type genotype (GG) was seen in 88.8% (127) of OSCC patients in comparison to 87.3% (69) controls; while variant genotypes (GA & AA) were seen in 9.8% (14) and 1.4% (2) of cases and 11.4% (9) and 1.3% (1) of controls respectively. Also no significant association was observed between variant genotypes (GA & AA) and oral cancer risk. Polymorphism of TNF-α at position −308 G/A may not be a risk factor for oral cancer because we did not find a statistically significant association between the TNF-α −308 G/A polymorphism and oral cancer risk (p = .710 and p = .946 for GA and AA respectively).
In conclusion, no association was seen between TNF-α −308 G/A polymorphism and oral cancer risk among the Malaysian Indian and Indigenous population. |
| format | Thesis |
| id | my.um.stud-3790 |
| institution | Universiti Malaya |
| publishDate | 2011 |
| record_format | eprints |
| spelling | my.um.stud-37902013-09-20T08:49:34Z Association between TNF-a-308 G/A polymorphism and oral cancer risk among Malaysian Indian and indigenous / Mojtaba Golpich Golpich, Mojtaba QH301 Biology The primary role of tumor necrosis factor alpha (TNF-α) gene is to regulate immune cells. Dysregulation and, in particular, overproduction of this gene has been found to increase susceptibility to a variety of human diseases such as cancer. The aim of this study is to investigate the association of single nucleotide polymorphism (SNP) in TNF-α −308 promoter and the risk of oral cancer among the Malaysian Indian and Indigenous population.The study included 143 confirmed oral squamous cell carcinoma (OSCC) (mean age = 63.69 ± 12.84) and 79 healthy controls (mean age = 50.43 ± 16.35). The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was employed to analyze TNF-α −308 promoter polymorphism, which were confirmed by direct sequencing. Chi-square, simple logistic regression and stratified analysis were performed using the SPSS (ver 15.0) to study the role of TNF-α polymorphism in modulating the risk of oral cancer. The wild-type genotype (GG) was seen in 88.8% (127) of OSCC patients in comparison to 87.3% (69) controls; while variant genotypes (GA & AA) were seen in 9.8% (14) and 1.4% (2) of cases and 11.4% (9) and 1.3% (1) of controls respectively. Also no significant association was observed between variant genotypes (GA & AA) and oral cancer risk. Polymorphism of TNF-α at position −308 G/A may not be a risk factor for oral cancer because we did not find a statistically significant association between the TNF-α −308 G/A polymorphism and oral cancer risk (p = .710 and p = .946 for GA and AA respectively). In conclusion, no association was seen between TNF-α −308 G/A polymorphism and oral cancer risk among the Malaysian Indian and Indigenous population. 2011 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/3790/1/1._Title_page%2C_abstract%2C_content.pdf application/pdf http://studentsrepo.um.edu.my/3790/2/2._Chapter_1_%E2%80%93_6.pdf application/pdf http://studentsrepo.um.edu.my/3790/3/3._References.pdf application/pdf http://studentsrepo.um.edu.my/3790/4/4._Appendices.pdf http://pendeta.um.edu.my/client/default/search/results?qu=Association+between+TNF-a-308+G%2FA+polymorphism+and+oral+cancer+risk&te= Golpich, Mojtaba (2011) Association between TNF-a-308 G/A polymorphism and oral cancer risk among Malaysian Indian and indigenous / Mojtaba Golpich. Masters thesis, University of Malaya. http://studentsrepo.um.edu.my/3790/ |
| spellingShingle | QH301 Biology Golpich, Mojtaba Association between TNF-a-308 G/A polymorphism and oral cancer risk among Malaysian Indian and indigenous / Mojtaba Golpich |
| title | Association between TNF-a-308 G/A polymorphism and oral cancer risk among Malaysian Indian and indigenous / Mojtaba Golpich |
| title_full | Association between TNF-a-308 G/A polymorphism and oral cancer risk among Malaysian Indian and indigenous / Mojtaba Golpich |
| title_fullStr | Association between TNF-a-308 G/A polymorphism and oral cancer risk among Malaysian Indian and indigenous / Mojtaba Golpich |
| title_full_unstemmed | Association between TNF-a-308 G/A polymorphism and oral cancer risk among Malaysian Indian and indigenous / Mojtaba Golpich |
| title_short | Association between TNF-a-308 G/A polymorphism and oral cancer risk among Malaysian Indian and indigenous / Mojtaba Golpich |
| title_sort | association between tnf-a-308 g/a polymorphism and oral cancer risk among malaysian indian and indigenous / mojtaba golpich |
| topic | QH301 Biology |
| url | http://studentsrepo.um.edu.my/3790/1/1._Title_page%2C_abstract%2C_content.pdf http://studentsrepo.um.edu.my/3790/2/2._Chapter_1_%E2%80%93_6.pdf http://studentsrepo.um.edu.my/3790/3/3._References.pdf http://studentsrepo.um.edu.my/3790/4/4._Appendices.pdf http://pendeta.um.edu.my/client/default/search/results?qu=Association+between+TNF-a-308+G%2FA+polymorphism+and+oral+cancer+risk&te= http://studentsrepo.um.edu.my/3790/ |
| url_provider | http://studentsrepo.um.edu.my/ |