PCR-Single strand conformation polymorphism (SSCP) analysis of Salmonella typhi and other Salmonella serovars
PCR-restriction fragment length polymorphism (PCR-RFLP) and PCR¨Csingle-strand conformation polymorphism(PCR-SSCP) analyses were carried out on the 1.6-kb groEL gene from 41 strains of 10 different Salmonella serovars. Three HaeIII RFLP profiles were recognized, but no discrimination between the ser...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | en |
| Published: |
American Society for Microbiology
2002
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/9966/1/Nair_et_al.%2C_2002.pdf http://eprints.um.edu.my/9966/ |
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| Summary: | PCR-restriction fragment length polymorphism (PCR-RFLP) and PCR¨Csingle-strand conformation polymorphism(PCR-SSCP) analyses were carried out on the 1.6-kb groEL gene from 41 strains of 10 different Salmonella serovars. Three HaeIII RFLP profiles were recognized, but no discrimination between the serovars could be achieved by this technique. However, PCR-SSCP analysis of the groEL genes of various Salmonella
serovars produced 14 SSCP profiles, indicating the potential of this technique to differentiate different Salmonella serovars (interserovar differentiation). Moreover, PCR-SSCP could differentiate strains within a subset of serovars (intraserovar discrimination), as three SSCP profiles were produced for the 11 Salmonella enterica serovar Enteritidis strains, and two SSCP profiles were generated for the 7 S. enterica serovar Infantis and five S. enterica serovar Newport strains. PCR-SSCP has the potential to complement classical typing methods such as serotyping and phage typing for the typing of Salmonella serovars due to its rapidity,
simplicity, and typeability. |
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