Technical improvement in DNA preparation for pulsed-field gel electrophoresis
We report a rapid pulsed-field gel electrophoresis (PFGE) protocol for subtyping Salmonella isolates. Modifications were made to reduce the number of steps and duration of proteolysis in our original protocol for the preparation of intact, genomic DNA for PFGE analysis. Comparison of PFGE patterns b...
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| Format: | Article |
| Language: | en |
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Faculty of Science, University of Malaya
2003
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| Online Access: | http://eprints.um.edu.my/9949/1/Thong%2C_Lai%2C_Goh%2C_2003.pdf http://eprints.um.edu.my/9949/ https://mjs.um.edu.my/index.php/MJS/article/view/8516 |
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| _version_ | 1831447970108669952 |
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| author | Thong, Kwai Lin Lai, K.S. Goh, Yee Ling |
| author_facet | Thong, Kwai Lin Lai, K.S. Goh, Yee Ling |
| author_sort | Thong, Kwai Lin |
| building | UM Library |
| collection | Institutional Repository |
| content_provider | Universiti Malaya |
| content_source | UM Research Repository |
| continent | Asia |
| country | Malaysia |
| description | We report a rapid pulsed-field gel electrophoresis (PFGE) protocol for subtyping Salmonella isolates. Modifications were made to reduce the number of steps and duration of proteolysis in our original protocol for the preparation of intact, genomic DNA for PFGE analysis. Comparison of PFGE patterns by independent researchers showed that subtyping results using this modified, rapid protocol were highly reproducible. |
| format | Article |
| id | my.um.eprints-9949 |
| institution | Universiti Malaya |
| language | en |
| publishDate | 2003 |
| publisher | Faculty of Science, University of Malaya |
| record_format | eprints |
| spelling | my.um.eprints-99492021-12-30T08:50:37Z http://eprints.um.edu.my/9949/ Technical improvement in DNA preparation for pulsed-field gel electrophoresis Thong, Kwai Lin Lai, K.S. Goh, Yee Ling QR Microbiology We report a rapid pulsed-field gel electrophoresis (PFGE) protocol for subtyping Salmonella isolates. Modifications were made to reduce the number of steps and duration of proteolysis in our original protocol for the preparation of intact, genomic DNA for PFGE analysis. Comparison of PFGE patterns by independent researchers showed that subtyping results using this modified, rapid protocol were highly reproducible. Faculty of Science, University of Malaya 2003 Article PeerReviewed application/pdf en http://eprints.um.edu.my/9949/1/Thong%2C_Lai%2C_Goh%2C_2003.pdf Thong, Kwai Lin and Lai, K.S. and Goh, Yee Ling (2003) Technical improvement in DNA preparation for pulsed-field gel electrophoresis. Malaysian Journal of Science, 22 (2). pp. 55-59. ISSN 1394-3065, https://mjs.um.edu.my/index.php/MJS/article/view/8516 |
| spellingShingle | QR Microbiology Thong, Kwai Lin Lai, K.S. Goh, Yee Ling Technical improvement in DNA preparation for pulsed-field gel electrophoresis |
| title | Technical improvement in DNA preparation for pulsed-field gel electrophoresis |
| title_full | Technical improvement in DNA preparation for pulsed-field gel electrophoresis |
| title_fullStr | Technical improvement in DNA preparation for pulsed-field gel electrophoresis |
| title_full_unstemmed | Technical improvement in DNA preparation for pulsed-field gel electrophoresis |
| title_short | Technical improvement in DNA preparation for pulsed-field gel electrophoresis |
| title_sort | technical improvement in dna preparation for pulsed-field gel electrophoresis |
| topic | QR Microbiology |
| url | http://eprints.um.edu.my/9949/1/Thong%2C_Lai%2C_Goh%2C_2003.pdf http://eprints.um.edu.my/9949/ https://mjs.um.edu.my/index.php/MJS/article/view/8516 |
| url_provider | http://eprints.um.edu.my/ |